biostudies-arrayexpress0000Michael BergerHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MEXP-3041Comparing gene expression profile of MLPC cultured in stem cell maintenance medium versus MLPC cultured in small airway growth mediumbiostudies-arrayexpressNucleic Acid Extraction - Total RNA was extracted using Tri Reagent, a phenol guanidinium isothiocyanate extraction method. The cells were lysed directly on the culture dish after removal of the culture medium. (Parameters: Extracted product = total_RNA, Amplification = none)Sample Processing - In this experiment, samples labelled as MB 6CA, MB C2 and MB C8 represent multilineage progenitor cells (MLPCs) isolated from human umbilical cord blood that were cultured in standard stem cell maintenance medium (Cambrex) for 8 days. Samples labelled as MB 5IA, MB I1 and MB I7 represent MLPCs that were cultured in small airway growth medium (SAGM)for 8 days, in this case functioning as a differentiation medium.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataAdditional FilesMAGE-TAB FilesArray DesignsAssay Data Transformation - Expressionist software (Genedata AG) was used for quality control of data. For each hybridization, the MAS 5.0 condensed data file was imported into the Expressionist Analyst. At a selected P value of <= 0.04, approximately 33,000 data points were significant. A summary box plot showed that the median values for all hybridizations were equal and therefore no normalization was deemed necessary.MetabolomicsUnknownTranscriptomicsGenomicsProteomicstranscription profiling by arrayHomo sapiensGene Expression Changes Accompanying Differentiation of Cord Blood Stem Cells Into Respiratory Epithelial Cells: A Preliminary ObservationMichael Berger0falseBerger MLPC to ATII gene expressionComparing gene expression profile of MLPC cultured in stem cell maintenance medium versus MLPC cultured in small airway growth medium2011-03-20T00:00:00Z2022-11-21T01:03:13.34Z2022-03-15T01:47:34.271ZE-MEXP-3041EFO_0002768