{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":[null],"study_type":["microRNA profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-10094"],"description":["We compared the variations of expression of miRNA/small nucleolar RNA (snoRNA) in a series of post-Chernobyl tumors developed in patients exposed to radioactive iodines, at thyroid doses below (21 to 48 mGy) or above (0·58 to 2·5 Gy) the limit of significance of conventional epidemiology, and in a series of unexposed Ukrainian patients. We identified miRNA/snoRNA discriminating signatures of thyroid PTC etiology in function of the dose range (“Low doses” or “High doses”) or independent of the dose (“Low+High doses”), as compared to sporadic tumors. Moreover, corresponding signatures of radiation exposure were also found analysing the non-pathologic tissues adjacent to the tumors of exposed and non-exposed patients, which showed an overlap with the etiology signatures."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA samples were prepared using RecoverAll Total Nucleic Acid Isolation kit (Ambion), according to the manufacturer’s protocol, with the exception of the proteinase K step. We use 6U per reaction of Proteinase K, recombinant, PCR grade (ThermoFisher scientific, France) instead of the Proteinase K provided by Ambion’s kit and incubation time was increased to 30mn, up to 60 min if needed, at 50°C to increase the RNA yield.  RNA quantity and quality was assessed by spectophotometry (260/280 and 260/230 ratio) using NanoDrop and by fluorometry using Qbit RNA High sensitivity (HS) and Qbit DNA HS assay (ThermoFisher scientific, France).RNA quality was verified using Agilent RNA6000 nano chip kit on a Bioanalyzer 2100 (Agilent Technologies, USA)","Hybridization - Labeled samples were hybridized to the arrays at 48°C for 20h. Hybridized arrays were washed and stained with a Streptavidin-PE solution","Sample Collection - sample collection protocol","Labeling - After validation of the RNA quality, 400 ng of total RNA was labeled using the FlashTag Biotin RNA Labeling Kit (Thermofisher, France) and hybridized to GeneChip miRNA 4.0 microarrays (Affymetrix Inc.) on the platform GENOM'IC (Cochin Institut, Paris, France),  as per the thermofisher guidelines. Briefly, 400 ng of RNA was poly-A tailed and a proprietary biotin-labeled dendramer molecule was joined to the 3′ end using DNA ligase.","Scaning - Chips were scanned using the GCS3000 7G to obtain raw data (cel files) and metrics for Quality Controls. Data were normalized using RMA algorithm in TAC software (Affymetrix)."],"figure_sub":["MIAME Score","Raw Data","Assays and Data","organisation","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Nicolas Ugolin","Catherine Ory"],"additional_accession":[]},"is_claimable":false,"name":"MiRNome/small RNA analysis of thyroid tumors and adjacent non tumoral tissues of Ukrainian patients exposed or not to Chernobyl radioiodines","description":"We compared the variations of expression of miRNA/small nucleolar RNA (snoRNA) in a series of post-Chernobyl tumors developed in patients exposed to radioactive iodines, at thyroid doses below (21 to 48 mGy) or above (0·58 to 2·5 Gy) the limit of significance of conventional epidemiology, and in a series of unexposed Ukrainian patients. We identified miRNA/snoRNA discriminating signatures of thyroid PTC etiology in function of the dose range (“Low doses” or “High doses”) or independent of the dose (“Low+High doses”), as compared to sporadic tumors. Moreover, corresponding signatures of radiation exposure were also found analysing the non-pathologic tissues adjacent to the tumors of exposed and non-exposed patients, which showed an overlap with the etiology signatures.","dates":{"release":"2026-02-24T00:00:00Z","modification":"2026-02-24T09:26:59.637Z","creation":"2022-02-03T23:35:17.54Z"},"accession":"E-MTAB-10094","cross_references":{"EFO":["EFO_0002944","EFO_0003814","EFO_0000753","EFO_0003813","EFO_0005518","EFO_0003815"]}}