{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Esten Nymoen Vandsemb"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-10437"],"description":["Phosphatase of Regenerating Liver 3 (PRL-3) is involved in oncogenesis in cancers of various tissues, but is only expressed at low levels in normal cells. To find processes and signaling pathways influenced by PRL-3, we overexpressed it using viral transduction in the JJN-3 cell line and compared the transcriptional changes to the mock transduced control cell line."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - The JJN-3 cells were grown in RPMI 1640 + 10 percent FCS. Seeded in T75 flask, 20 ml medium. Split 1:10 or 1:15 every 3 to 4 days, when density of cells is normally between 0.3-0.7 x 10E6 cells/ml","Nucleic Acid Extraction - RNeasy Mini Kit (Qiagen, Crawley, United Kingdom)","Sample Collection - Cell lines were pelleted by centrifugation (1500 RPM, 5 minutes)","Library Construction - SENSE mRNA-Seq library prep kit (Lexogen GmbH, Vienna, Austria)","Sequencing - Single read sequencing, 75 cycles on a NextSeq 500 instrument, according to the manufacturer's instructions"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Esten Nymoen Vandsemb"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of human multiple myeloma cell line JJN-3 virally transduced to overexpress PRL-3 compared to JJN-3 cells transduced with mock control","description":"Phosphatase of Regenerating Liver 3 (PRL-3) is involved in oncogenesis in cancers of various tissues, but is only expressed at low levels in normal cells. To find processes and signaling pathways influenced by PRL-3, we overexpressed it using viral transduction in the JJN-3 cell line and compared the transcriptional changes to the mock transduced control cell line.","dates":{"release":"2021-07-31T00:00:00Z","modification":"2022-03-10T07:07:27.829Z","creation":"2022-03-10T07:07:27.829Z"},"accession":"E-MTAB-10437","cross_references":{"ENA":["ERP128887"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003738","EFO_0004184"]}}