biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsEsten Nymoen VandsembNextSeq 500RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-10437Phosphatase of Regenerating Liver 3 (PRL-3) is involved in oncogenesis in cancers of various tissues, but is only expressed at low levels in normal cells. To find processes and signaling pathways influenced by PRL-3, we overexpressed it using viral transduction in the JJN-3 cell line and compared the transcriptional changes to the mock transduced control cell line.biostudies-arrayexpressGrowth Protocol - The JJN-3 cells were grown in RPMI 1640 + 10 percent FCS. Seeded in T75 flask, 20 ml medium. Split 1:10 or 1:15 every 3 to 4 days, when density of cells is normally between 0.3-0.7 x 10E6 cells/mlNucleic Acid Extraction - RNeasy Mini Kit (Qiagen, Crawley, United Kingdom)Sample Collection - Cell lines were pelleted by centrifugation (1500 RPM, 5 minutes)Library Construction - SENSE mRNA-Seq library prep kit (Lexogen GmbH, Vienna, Austria)Sequencing - Single read sequencing, 75 cycles on a NextSeq 500 instrument, according to the manufacturer's instructionsOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesEsten Nymoen VandsembfalseRNA-seq of human multiple myeloma cell line JJN-3 virally transduced to overexpress PRL-3 compared to JJN-3 cells transduced with mock controlPhosphatase of Regenerating Liver 3 (PRL-3) is involved in oncogenesis in cancers of various tissues, but is only expressed at low levels in normal cells. To find processes and signaling pathways influenced by PRL-3, we overexpressed it using viral transduction in the JJN-3 cell line and compared the transcriptional changes to the mock transduced control cell line.2021-07-31T00:00:00Z2022-03-10T07:07:27.829Z2022-03-10T07:07:27.829ZE-MTAB-10437ERP128887EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184