biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMingxia LiIllumina NovaSeq 6000ChIP-seqMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-10585The deubiquitinating enzyme BAP1 can remove the ubiquitination modification of H2AK119. To reveal the mechanism by which Bap1 deletion in  MC38 colon carcinoma cell line causes an anti-tumor immune response from the epigenetic level, we generated CUT&Tag data of H2AK119Ub, H3K27me3, H3K27ac and H3K4me3 histone in MC38-Bap1-Wildtype and MC38-Bap1-Knockout isolated from Rag2-deficient mice.biostudies-arrayexpressSequencing - Then Illumina platform sequencing were accomplished according to Illumina instructions.Nucleic Acid Extraction - CUT&Tag experiment was performed according to the reaction system recommended by Vazyme #TD901-2 follow the instructions.Library Construction - To stop the fragmentation reaction, 10 μl 0.5M EDTA,3 μl 10% SDS and 2.5 μl 20 mg/ml Proteinase K were incubated with mixture overnight at 37℃. 150 μl Tris saturated phenol and 150 μl chloroform were added and centrifuge for 5 min at 16,000 g to collect the supernatant. Add 300 μl chloroform and centrifuge to collect the supernatant, then which was added to 750 μl 100% ethanol in tube on ice, and centrifuge for 15 min at 1,6000 g at 4℃to collect the DNA. Last sample was washed using 100% ethanol, and dissolved in double-distilled water. For library construction, set up the PCR reaction as follows: for a total volume of 50 μL, add 24 μL of DNA,5 μL of ddH2O,10 μL of 5 × TAB buffer, 5μL P5 primer X, 5 μL P7 primer X, and 1 μL of TAE. Mix gently and spin briefly. Then PCR amplification program were carried out,Sample Collection - Tumor cells expressing BFP fluorescent protein were isolated from Rag2-/- mice, Tumor Dissociation Kit (Miltenyi,130-096-730) was used to dissociate the tumor tissues into single cells as the manufacturer’s instructions. ACK lysis buffer (Gibco,492-01) was used to remove the red blood cell. Then Dead Cell Removal kit (Miltenyi,130-090-101) was used to remove dead cells by column in MACS Separator,then Then the tumor cells were resuspended with Facs buffer and BFP+ tumor were sorted at 4℃.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMingxia LifalseCUT&Tag chromatin profiling of H2AK119Ub, H3K27me3, H3K27ac and H3K4me3 histone in  MC38 colon carcinoma cell lineThe deubiquitinating enzyme BAP1 can remove the ubiquitination modification of H2AK119. To reveal the mechanism by which Bap1 deletion in  MC38 colon carcinoma cell line causes an anti-tumor immune response from the epigenetic level, we generated CUT&Tag data of H2AK119Ub, H3K27me3, H3K27ac and H3K4me3 histone in MC38-Bap1-Wildtype and MC38-Bap1-Knockout isolated from Rag2-deficient mice.2025-07-06T00:00:00Z2023-07-10T15:48:17.372Z2023-07-10T15:48:17.372ZE-MTAB-10585ERP149369E-MTAB-10588E-MTAB-10610E-MTAB-10587E-MTAB-10593EFO_0002944EFO_0004170EFO_0002692EFO_0005518EFO_0004184