biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMingxia LiIllumina NovaSeq 6000ATAC-seqMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-10593Genome-wide measurements of chromatin accessibility in MC38 colon carcinoma cells and MC38 Bap1-knockout cells.biostudies-arrayexpressSequencing - Libraries were sequenced on Illumina NovaSeq 6000 machines with 150 bp pair end mode. Adapters in the reads were trimmed using fastp (PMID 30423086) with the “--detect_adapter_for_pe” flag.Library Construction - Adapters in the reads were trimmed using fastp (PMID 30423086) with the “--detect_adapter_for_pe” flag. The trimmed reads were aligned to the mouse reference genome (mm10) using hisat2 (PMID 3137580) with the “--no-temp-splicesite --no-spliced-alignment” flags.Nucleic Acid Extraction - The DNA in the nucles was processed by transposition reaction using Tn5 Transposase and purified follow the instructions.Sample Collection - After the 1000u/ml IFN-β stimulation for 48 hours, the tumor were collected and lysed in order to extract the cell nucleus.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMingxia LifalseATAC-Seq analysis of MC38 colon carcinoma cells and MC38 Bap1-knockout cellsGenome-wide measurements of chromatin accessibility in MC38 colon carcinoma cells and MC38 Bap1-knockout cells.2025-07-06T00:00:00Z2023-07-10T16:30:55.446Z2023-07-10T16:30:55.446ZE-MTAB-10593ERP149375E-MTAB-10588E-MTAB-10610E-MTAB-10587E-MTAB-10585E-MTAB-10584EFO_0002944EFO_0007045EFO_0004170EFO_0005518EFO_0004184