biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMingxia LiIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-10610Here, we used single cell RNA-sequencing (scRNA-seq) to profile the difference of the CD45 positive cells subgroups infiltrating in MC38-Bap1-Wildtype and MC38-Bap1-Knockout tumor microenvironment on the 10th day of tumor growth in C57BL/6 mice. Additionally, we used scRNA-seq to profile differential gene expression of CD8 T cells and the difference of related signal.biostudies-arrayexpressLibrary Construction - Sequencing library was constructed according to the instructions of Chromium Single Cell 3ʹ Reagent kits v3 (10×Genomics).Nucleic Acid Extraction - The cell suspension was loaded into Chromium microfluidic chips with 30v3 chemistry and barcoded with a 10× Chromium Controller (10X Genomics). mRNA from the barcoded cells was reverse-transcribed into cDNA according to the manufacturer's instructions .Sample Collection - Tumor cells were separated from the C57BL/6 mice on the 10th of tumor growth, Tumor Dissociation Kit (Miltenyi,130-096-730) was used to dissociate the tumor tissues into single cells as the manufacturer’s instructions. ACK lysis buffer (Gibco,492-01) was used to remove the red blood cell. Then Dead Cell Removal kit (Miltenyi,130-090-101) was used to remove dead cells by column in MACS Separator, and CD45+ cells were enriched from tumor tissues according to CD45(TIL) MicroBeads (Miltenyi,130-110-618).The cells were resuspended with 1×PBS containing 0.04% BSA, and cell viability was detected with 0.4% trypan blue staining solution and counted with Countess II (Invitrogen) cell counter, and the cell viability was over 90%.Sequencing - Sequencing was performed with NovaSeq according to Illumina instructions .OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMingxia LifalseSingle cell analysis of CD45 positive cells isolated from MC38 colon carcinoma tumor-bearing C57BL/6 miceHere, we used single cell RNA-sequencing (scRNA-seq) to profile the difference of the CD45 positive cells subgroups infiltrating in MC38-Bap1-Wildtype and MC38-Bap1-Knockout tumor microenvironment on the 10th day of tumor growth in C57BL/6 mice. Additionally, we used scRNA-seq to profile differential gene expression of CD8 T cells and the difference of related signal.2025-06-29T00:00:00Z2023-07-07T11:00:20.845Z2022-03-10T04:33:16.715ZE-MTAB-10610ERP129735EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184