biostudies-arrayexpressNianshu ZhangSaccharomyces cerevisiae S288Chttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-11024To find how the Mck1 and Slt2 kinases regulate metabolic reprogramming in response to glucose starvation in Saccharomyces cerevisiae cells, transcriptome of wild-type cells, single and double deletion mutants of MCK1 and SLT2 grown at both glucose-replete (exponential phase) and glucose-starved (early PDS (post-diauxic shift) phase) conditions were determined.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated from using an RNeasy Mini kit (Qiagen, 74104).Sequencing - The qualified libraries are fed into Illumina sequencers after pooling according to its effective concentration and expected data volume.Sample Collection - 2X10(7)exponentially growing (5h post-inoculation) and early post-diauxic phase (13h post-inoculation) cells were harvested by centrifugation at 1000 x g for 5 min at 4°C.Library Construction - After the QC procedures of total RNA, mRNA is enriched using oligo(dT) beads. Enriched mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is first synthesized by using random hexamers primer, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - DESeq2 (version 1.24.0) analyses (Love et al. 2014) were performed within R (version 3.6.1), for normalisation and comparing the changes in gene expression.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 2500RNA-seq of coding RNASaccharomyces cerevisiae S288CNianshu ZhangfalseRNA-seq of wild-type cells, single and double mutants of MCK1 and SLT2 grown at exponential and early-diauxic shift phasesTo find how the Mck1 and Slt2 kinases regulate metabolic reprogramming in response to glucose starvation in Saccharomyces cerevisiae cells, transcriptome of wild-type cells, single and double deletion mutants of MCK1 and SLT2 grown at both glucose-replete (exponential phase) and glucose-starved (early PDS (post-diauxic shift) phase) conditions were determined.2025-05-31T00:00:00Z2024-12-02T14:00:57.897Z2022-03-14T18:23:27.015ZE-MTAB-11024ERP132296EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184