{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Gabriele Lubatti"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-11835"],"description":["study of the heterogeneity of mammalian pluripotent stem cell lines"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Chromium Singe Cell 3’ v3, 10x Genomics","Sample Collection - Cells were trypsinized and FACS sorted to select live and single cells. Cells were washed twice and passed through a cell strainer 35um before final counting and viability assessment with an automatic cell counter.","Sequencing - NovaSeq6000. Paired-end 100bp sequencing","Sample Collection - The mouse iPSC were a gift from Hans Schöler lab: Velychko, S., Adachi, K., Kim, K.-P., Hou, Y., MacCarthy, C.M., Wu, G., and Schöler, H.R. (2019). Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem Cellhttps://doi.org/10.1016/j.stem.2019.10.002.ROSA26-rtTA/GOF18Briefly, mouse embryonic fibroblasts of passage 3 or 4 were reprogrammed by lentiviral infection with a dox inducible cassette expressing Sox2/Klf4/c-Myc. Strain is a mixed background C57BL/6 and 129. Cells were trypsinized and FACS sorted to select live and single cells and to exclude feeder cells colored in red. Cells were washed twice and passed through a cell strainer 35um before final counting and viability assessment with an automatic cell counter.","Nucleic Acid Extraction - Chromium Singe Cell 3’ v3, 10x Genomics"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Gabriele Lubatti"],"additional_accession":[]},"is_claimable":false,"name":"Study of the heterogeneity of mammalian pluripotent stem cell lines (Mouse ESCs and iPSCs)","description":"study of the heterogeneity of mammalian pluripotent stem cell lines","dates":{"release":"2025-12-30T00:00:00Z","modification":"2025-12-30T02:02:06.286Z","creation":"2022-07-08T13:00:14.731Z"},"accession":"E-MTAB-11835","cross_references":{"ENA":["ERP139450"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}