biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsGabriele LubattiIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-11835study of the heterogeneity of mammalian pluripotent stem cell linesbiostudies-arrayexpressLibrary Construction - Chromium Singe Cell 3’ v3, 10x GenomicsSample Collection - Cells were trypsinized and FACS sorted to select live and single cells. Cells were washed twice and passed through a cell strainer 35um before final counting and viability assessment with an automatic cell counter.Sequencing - NovaSeq6000. Paired-end 100bp sequencingSample Collection - The mouse iPSC were a gift from Hans Schöler lab: Velychko, S., Adachi, K., Kim, K.-P., Hou, Y., MacCarthy, C.M., Wu, G., and Schöler, H.R. (2019). Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem Cellhttps://doi.org/10.1016/j.stem.2019.10.002.ROSA26-rtTA/GOF18Briefly, mouse embryonic fibroblasts of passage 3 or 4 were reprogrammed by lentiviral infection with a dox inducible cassette expressing Sox2/Klf4/c-Myc. Strain is a mixed background C57BL/6 and 129. Cells were trypsinized and FACS sorted to select live and single cells and to exclude feeder cells colored in red. Cells were washed twice and passed through a cell strainer 35um before final counting and viability assessment with an automatic cell counter.Nucleic Acid Extraction - Chromium Singe Cell 3’ v3, 10x GenomicsOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesGabriele LubattifalseStudy of the heterogeneity of mammalian pluripotent stem cell lines (Mouse ESCs and iPSCs)study of the heterogeneity of mammalian pluripotent stem cell lines2025-12-30T00:00:00Z2025-12-30T02:02:06.286Z2022-07-08T13:00:14.731ZE-MTAB-11835ERP139450EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184