{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Gabriele Lubatti"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA from single cells"],"organism":["Oryctolagus cuniculus"],"species":["Oryctolagus cuniculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-11916"],"description":["study of the heterogeneity of mammalian pluripotent stem cell lines"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Chromium Singe Cell 3’ v3.1, 10x Genomics","Library Construction - Chromium Singe Cell 3’ v3, 10x Genomics","Sample Collection - Cells were trypsinized then washed twice and passed through a cell strainer 35um before final counting and viability assessement was done manually.","Sample Collection - rabbit ESCs : AKSL20 ESCs, paper :Osteil, P., Moulin, A., Santamaria, C., Joly, T., Jouneau, L., Aubry, M., Tapponnier, Y., Archilla, C., Schmaltz-Panneau, B., Lecardonnel, J., et al. (2016). A Panel of Embryonic Stem Cell Lines Reveals the Variety and Dynamic of Pluripotent States in Rabbits. Stem Cell Reports 7, 383–398. https://doi.org/10.1016/j.stemcr.2016.07.022. rabbit iPSCs: rbEKA9 iPSCs, comes from ear skin fibroblasts: Tapponnier, Y., Afanassieff, M., Aksoy, I., Aubry, M., Moulin, A., Medjani, L., Bouchereau, W., Mayère, C., Osteil, P., Nurse-Francis, J., et al. (2017). Reprogramming of rabbit induced pluripotent stem cells toward epiblast and chimeric competency using Krüppel-like factors. Stem Cell Research 24, 106–117. https://doi.org/10.1016/j.scr.2017.09.001. Cells were trypsinized and FACS sorted to select life and single cells and to exclude feeder cells colored in red.  Cells were washed twice and passed through a cell strainer 35um before final counting and viability assessement with an automatic cell counter.","Sequencing - NovaSeq6000. Paired-end 150bp sequencing","Sequencing - NovaSeq6000. Paired-end 100bp sequencing","Library Construction - Chromium Singe Cell 3’ v3.1, 10x Genomics","Nucleic Acid Extraction - Chromium Singe Cell 3’ v3, 10x Genomics"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Gabriele Lubatti"],"additional_accession":[]},"is_claimable":false,"name":"Study of the heterogeneity of mammalian pluripotent stem cell lines (Rabbit ESCs and iPSCs)","description":"study of the heterogeneity of mammalian pluripotent stem cell lines","dates":{"release":"2025-12-30T00:00:00Z","modification":"2025-12-30T02:02:06.593Z","creation":"2022-08-01T18:34:56.523Z"},"accession":"E-MTAB-11916","cross_references":{"ENA":["ERP139721"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0004184"]}}