biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsGabriele LubattiIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsOryctolagus cuniculusOryctolagus cuniculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-11916study of the heterogeneity of mammalian pluripotent stem cell linesbiostudies-arrayexpressNucleic Acid Extraction - Chromium Singe Cell 3’ v3.1, 10x GenomicsLibrary Construction - Chromium Singe Cell 3’ v3, 10x GenomicsSample Collection - Cells were trypsinized then washed twice and passed through a cell strainer 35um before final counting and viability assessement was done manually.Sample Collection - rabbit ESCs : AKSL20 ESCs, paper :Osteil, P., Moulin, A., Santamaria, C., Joly, T., Jouneau, L., Aubry, M., Tapponnier, Y., Archilla, C., Schmaltz-Panneau, B., Lecardonnel, J., et al. (2016). A Panel of Embryonic Stem Cell Lines Reveals the Variety and Dynamic of Pluripotent States in Rabbits. Stem Cell Reports 7, 383–398. https://doi.org/10.1016/j.stemcr.2016.07.022. rabbit iPSCs: rbEKA9 iPSCs, comes from ear skin fibroblasts: Tapponnier, Y., Afanassieff, M., Aksoy, I., Aubry, M., Moulin, A., Medjani, L., Bouchereau, W., Mayère, C., Osteil, P., Nurse-Francis, J., et al. (2017). Reprogramming of rabbit induced pluripotent stem cells toward epiblast and chimeric competency using Krüppel-like factors. Stem Cell Research 24, 106–117. https://doi.org/10.1016/j.scr.2017.09.001. Cells were trypsinized and FACS sorted to select life and single cells and to exclude feeder cells colored in red. Cells were washed twice and passed through a cell strainer 35um before final counting and viability assessement with an automatic cell counter.Sequencing - NovaSeq6000. Paired-end 150bp sequencingSequencing - NovaSeq6000. Paired-end 100bp sequencingLibrary Construction - Chromium Singe Cell 3’ v3.1, 10x GenomicsNucleic Acid Extraction - Chromium Singe Cell 3’ v3, 10x GenomicsOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesGabriele LubattifalseStudy of the heterogeneity of mammalian pluripotent stem cell lines (Rabbit ESCs and iPSCs)study of the heterogeneity of mammalian pluripotent stem cell lines2025-12-30T00:00:00Z2025-12-30T02:02:06.593Z2022-08-01T18:34:56.523ZE-MTAB-11916ERP139721EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184