biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsGabriele LubattiIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsBos taurusBos taurushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-11994study of the heterogeneity of mammalian pluripotent stem cell linesbiostudies-arrayexpressNucleic Acid Extraction - Chromium Singe Cell 3’ v3.1, 10x GenomicsSample Collection - Bovine embryonic stem cells were described in the following publication: Kinoshita, M., Kobayashi, T., Planells, B., Klisch, D., Spindlow, D., Masaki, H., Bornelöv, S., Stirparo, G.G., Matsunari, H., Uchikura, A., et al. (2021). Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species. Development 148, dev199901. https://doi.org/10.1242/dev.199901. Cells were trypsinized then washed twice and passed through a cell strainer 35um before final counting and viability assessement was done manually.Library Construction - Chromium Singe Cell 3’ v3.1, 10x GenomicsSequencing - NovaSeq6000. Paired-end 100bp sequencingOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesGabriele LubattifalseStudy of the heterogeneity of mammalian pluripotent stem cell lines (Cow ESCs)study of the heterogeneity of mammalian pluripotent stem cell lines2025-12-30T00:00:00Z2025-12-30T02:02:05.785Z2022-08-01T18:36:21.183ZE-MTAB-11994ERP139790EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184