{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Gabriele Lubatti"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA from single cells"],"organism":["Sus scrofa"],"species":["Sus scrofa"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-11996"],"description":["study of the heterogeneity of mammalian pluripotent stem cell lines"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Chromium Singe Cell 3’ v3.1, 10x Genomics","Library Construction - Chromium Singe Cell 3’ v3, 10x Genomics","Sample Collection - Cells were trypsinized then washed twice and passed through a cell strainer 35um before final counting and viability assessement was done manually.","Sample Collection - Cells were treated with collagenase to detach colonies from feeders, and the supernatant was collected. Cells were then trypsinized for 2 minutes before washing the pellet twice and passing them through a 35um cell strainer. Final counting and viability assessement was done with an automatic cell counter.","Sample Collection - Cells were trypsinized then washed twice and passed through a cell strainer 35um before final counting and viability assessement with an automatic cell counter.","Sequencing - NovaSeq6000. Paired-end 150bp sequencing","Sequencing - NovaSeq6000. Paired-end 100bp sequencing","Library Construction - Chromium Singe Cell 3’ v3.1, 10x Genomics","Nucleic Acid Extraction - Chromium Singe Cell 3’ v3, 10x Genomics"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Gabriele Lubatti"],"additional_accession":[]},"is_claimable":false,"name":"Study of the heterogeneity of mammalian pluripotent stem cell lines (Pig ESCs and iPSCs)","description":"study of the heterogeneity of mammalian pluripotent stem cell lines","dates":{"release":"2025-12-30T00:00:00Z","modification":"2025-12-30T02:02:05.95Z","creation":"2022-08-01T18:36:01.481Z"},"accession":"E-MTAB-11996","cross_references":{"ENA":["ERP139808"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0004184"]}}