biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsGabriele LubattiIllumina NovaSeq 6000RNA-seq of coding RNA from single cellsSus scrofaSus scrofahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-11996study of the heterogeneity of mammalian pluripotent stem cell linesbiostudies-arrayexpressNucleic Acid Extraction - Chromium Singe Cell 3’ v3.1, 10x GenomicsLibrary Construction - Chromium Singe Cell 3’ v3, 10x GenomicsSample Collection - Cells were trypsinized then washed twice and passed through a cell strainer 35um before final counting and viability assessement was done manually.Sample Collection - Cells were treated with collagenase to detach colonies from feeders, and the supernatant was collected. Cells were then trypsinized for 2 minutes before washing the pellet twice and passing them through a 35um cell strainer. Final counting and viability assessement was done with an automatic cell counter.Sample Collection - Cells were trypsinized then washed twice and passed through a cell strainer 35um before final counting and viability assessement with an automatic cell counter.Sequencing - NovaSeq6000. Paired-end 150bp sequencingSequencing - NovaSeq6000. Paired-end 100bp sequencingLibrary Construction - Chromium Singe Cell 3’ v3.1, 10x GenomicsNucleic Acid Extraction - Chromium Singe Cell 3’ v3, 10x GenomicsOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesGabriele LubattifalseStudy of the heterogeneity of mammalian pluripotent stem cell lines (Pig ESCs and iPSCs)study of the heterogeneity of mammalian pluripotent stem cell lines2025-12-30T00:00:00Z2025-12-30T02:02:05.95Z2022-08-01T18:36:01.481ZE-MTAB-11996ERP139808EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184