biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsTao WangIllumina HiSeq 4000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-12142The mesoderm is important in driving pulmonary organogenesis. We described a new method for rapid producing pulmonary organoids from human induced pluripotent stem cells (iPSCs) by co-culturing anterior foregut endoderm (AFE) and mesoderm progenitors. The pulmonary organoids spontaneously formed within 14 days of the co-culturing. We measured gene expression on the pulmonary organoids and normal human iPSCs using RNAseq.biostudies-arrayexpressLibrary Construction - Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.Sequencing - Sequenced paired end 76bp on HISEQ4000 at the Genomic Technologies Core Facility University of Manchester.Nucleic Acid Extraction - The pulmonary organoids were first treated with Corning cell recovery solution (Corning, Deeside, UK) to recover cells, and then the total RNAs were extracted using TRIZOL and phenol-chloroform. Total RNA from iPSCs was extracted using the RNeasy Mini Kit (Qiagen).Sample Collection - 4 iPSC lines used are: SOJD3, SEUR7, WT02C3 and WT02C9. nto anterior foregut endoderm and mesoderm progenitors. The two population of cells were imbedded in Matrigel and co-cultured in an insert of a 12 mm Transwell that was emerged in DMEM base medium supplemented with human serum replacement 3, BMP4, FGF2, FGF7, and FGF10. The medium was changes every 2-3 days and the pulmonary organoids spontaneously formed within 14 days of the co-culture. The pulmonary organoids were first treated with Corning cell recovery solution (Corning, Deeside, UK) to recover cells before nucleic RNA extraction. The induced pluripotent stem cells were cultured in Essential 8 Medium (Thermo Fisher Scientific)OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesTao WangRachel ScholeyfalseRNAseq of iPSC derived pulmonary organoids by co-culturing endodermal and mesodermal progenitorsThe mesoderm is important in driving pulmonary organogenesis. We described a new method for rapid producing pulmonary organoids from human induced pluripotent stem cells (iPSCs) by co-culturing anterior foregut endoderm (AFE) and mesoderm progenitors. The pulmonary organoids spontaneously formed within 14 days of the co-culturing. We measured gene expression on the pulmonary organoids and normal human iPSCs using RNAseq.2025-08-01T00:00:00Z2025-08-02T00:01:51.265Z2022-10-12T09:20:48.069ZE-MTAB-12142ERP141587EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184