{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-12479"],"description":["The study involves whole transcriptome sequencing of 30 orphan FFPE tumors obtained from anaplastic thyroid carcinoma patients of Indian origin. With this, we aim to  describe the expression profile, fusion genes and pathogen profile of this specific subset of anaplastic thyroid cancer patients. This knowledge will further allow us to gain an insight into transcriptomic alterations prevalent in Indian anaplastic thyroid carcinoma."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - The FFPE tumor tissues were collected from ACTREC-TMC","Sequencing - 30 samples' whole transcriptome libraries were put into the Novaseq 6000 platform at 4basecare Pvt. Ltd., Bangalore, for 2 X 150 bp paired-end sequencing","Library Construction - Using the Illumina RNA Prep with enrichment Kit, formerly known as the TruSeq RNA Access Library Prep Kit, whole transcriptome sequencing was done on 30 FFPE samples of anaplastic thyroid cancer.","Nucleic Acid Extraction - The 30 FFPE orphan tumor samples has been subjected to microtome-sectioning where ~5-6 sections of 10 um has been taken based on the tissue abundance for RNA extraction. The RNA extraction from has been performed according to standard protocol of Qiagen's RNeasy FFPE tissue kits. The RNA concentration was measured using Nanodrop 2000c spectrophotometer."],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"additional_accession":["ERP143399"],"pubmed_authors":["Amit Dutt"]},"is_claimable":false,"name":"Whole transcriptome sequencing of anaplastic thyroid cancer of Indian origin","description":"The study involves whole transcriptome sequencing of 30 orphan FFPE tumors obtained from anaplastic thyroid carcinoma patients of Indian origin. With this, we aim to  describe the expression profile, fusion genes and pathogen profile of this specific subset of anaplastic thyroid cancer patients. This knowledge will further allow us to gain an insight into transcriptomic alterations prevalent in Indian anaplastic thyroid carcinoma.","dates":{"release":"2025-11-09T00:00:00Z","modification":"2025-11-09T02:01:46.592Z","creation":"2023-01-06T14:23:58.821Z"},"accession":"E-MTAB-12479","cross_references":{"ENA":["ERP143399"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}