biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMariella FerranteNextSeq 550RNA-seq of coding RNAPseudo-nitzschia multistriataPseudo-nitzschia multistriatahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-12676The marine diatom Pseudo-nitzschia multistriata has a heterothallic life cycle, with two opposite mating types (MTs). As many diatoms, P. multistriata cells undergo cell size reduction at each mitotic division. Young, large cells above a certain size threshold are unable to reproduce sexually, they have to reach a critical size called sexualization size threshold (SST) and below this SST become capable to undergo meiosis, when encountering a cell of opposite MT. This experiment aims to provide a transcriptomic dataset which includes data from cells of different size (below and above the SST) and of different MT, to characterize the gene regulatory network involved in the sexualization process.biostudies-arrayexpressSequencing - The pooled samples were subject to cluster generation and sequencing using an Illumina NextSeq 550 Dx System (Illumina) in a 1x75 single read format at a final concentration of 1.8 pmol. The raw sequence files generated (.fastq files) underwent quality control analysis using FastQ.Next generation sequencing experiments were performed by Genomix4life S.R.L. (Baronissi, Salerno, Italy)Nucleic Acid Extraction - RNA was extracted with the Trizol RNA isolation protocolLibrary Construction - RNA concentration in each sample was assayed with a ND-1000 spectrophotometer (NanoDrop) and its quality assessed with the TapeStation 4200 (Agilent Technologies). Indexed libraries were prepared from 800 ng/ea purified RNA with TruSeq Stranded mRNA Sample Prep Kit (Illumina) according to the manufacturer’s instructions. Libraries were quantified using the TapeStation 4200 (Agilent Technologies) and and Qubit fluorometer (Invitrogen Co.), then pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 2nM.Sample Collection - Cultures were collected by centrifugation (4000rpm @18° for 15 minutes). 1 ml of Trizol reagent was added to dry pellet.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesAntonella RuggieroMariella FerrantefalseComparison of Pseudo-nitzschia multistriata cells of opposite mating type above and below the sexualization size thresholdThe marine diatom Pseudo-nitzschia multistriata has a heterothallic life cycle, with two opposite mating types (MTs). As many diatoms, P. multistriata cells undergo cell size reduction at each mitotic division. Young, large cells above a certain size threshold are unable to reproduce sexually, they have to reach a critical size called sexualization size threshold (SST) and below this SST become capable to undergo meiosis, when encountering a cell of opposite MT. This experiment aims to provide a transcriptomic dataset which includes data from cells of different size (below and above the SST) and of different MT, to characterize the gene regulatory network involved in the sexualization process.2026-06-22T00:00:00Z2026-06-22T18:10:52.335Z2023-02-17T16:04:41.266ZE-MTAB-12676ERP145096EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184