{"database":"biostudies-arrayexpress","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":9,"searchCount":0},"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Thorsten Stiewe"],"study_type":["transcription profiling by array"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-1278"],"description":["HS766T human pancreatic carcinoma cells endogenously express high levels of p73. Here these cells were transfected with siRNAs targeting p73 to analyze the p73-dependent expression profile."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Processing - Cells were transfected with p73-specific siRNA or non-targeting siRNA and RNA was isolated after 72h.","Labeling - 100ng total RNA were amplified and labeled using the Agilent Quick Amp Labeling Kit (Agilent, 5190-0444)","Nucleic Acid Extraction - Total RNA was extracted using the RNeasy Mini Kit (Qiagen)","Hybridization - SurePrint G3 Human GE 8x60K Microarray (Design ID: 039494) (Agilent Technologies) was used for the analysis of the gene expression of the different samples in a reference design assay.","Growth Protocol - Cells were cultured under standard conditions"],"pubmed_title":["p73 drives an oncogenic transcription program essential for tumor cell proliferation"],"data_protocol":["Image Adquisition - Scanning was performed using an Agilent DNA microarray scanner G2505C (scan software: Agilent Scan Control version A.8.1.3; quantification software: Agilent Feature Extraction version 10.5.1.1 (FE Protocol GE2_105_Dec08))."],"pubmed_authors":["Katharina Schlereth, Lukas Rycak and Thorsten Stiewe","Thorsten Stiewe"],"view_count":["9"],"additional_accession":[]},"is_claimable":false,"name":"Transcription profiling by array of HS766T human pancreatic carcinoma cells transfected with siRNAs targeting p73 to analyze the p73-dependent expression profile","description":"HS766T human pancreatic carcinoma cells endogenously express high levels of p73. Here these cells were transfected with siRNAs targeting p73 to analyze the p73-dependent expression profile.","dates":{},"accession":"E-MTAB-1278","cross_references":{"EFO":["EFO_0002768"]}}