biostudies-arrayexpress0090MetabolomicsUnknownTranscriptomicsGenomicsProteomicsThorsten Stiewetranscription profiling by arrayHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-1278HS766T human pancreatic carcinoma cells endogenously express high levels of p73. Here these cells were transfected with siRNAs targeting p73 to analyze the p73-dependent expression profile.biostudies-arrayexpressSample Processing - Cells were transfected with p73-specific siRNA or non-targeting siRNA and RNA was isolated after 72h.Labeling - 100ng total RNA were amplified and labeled using the Agilent Quick Amp Labeling Kit (Agilent, 5190-0444)Nucleic Acid Extraction - Total RNA was extracted using the RNeasy Mini Kit (Qiagen)Hybridization - SurePrint G3 Human GE 8x60K Microarray (Design ID: 039494) (Agilent Technologies) was used for the analysis of the gene expression of the different samples in a reference design assay.Growth Protocol - Cells were cultured under standard conditionsp73 drives an oncogenic transcription program essential for tumor cell proliferationImage Adquisition - Scanning was performed using an Agilent DNA microarray scanner G2505C (scan software: Agilent Scan Control version A.8.1.3; quantification software: Agilent Feature Extraction version 10.5.1.1 (FE Protocol GE2_105_Dec08)).Katharina Schlereth, Lukas Rycak and Thorsten StieweThorsten Stiewe9falseTranscription profiling by array of HS766T human pancreatic carcinoma cells transfected with siRNAs targeting p73 to analyze the p73-dependent expression profileHS766T human pancreatic carcinoma cells endogenously express high levels of p73. Here these cells were transfected with siRNAs targeting p73 to analyze the p73-dependent expression profile.E-MTAB-1278EFO_0002768