{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["YANG JIANG"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["ATAC-seq"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-12903"],"description":["To test the chromatin accessibility of different immune cell subsets, we isolated naive B cells CD11c+T-bet+ B cells,  CD11c-T-bet- B cells by flow cytometry from Bm12-induced lupus T-bet reporter mice and performed ATAC assay."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Fragmented DNA was purified using VAHTS DNA Clean Beads and library was generated by PCR.","Sample Collection - bm12-cGVHD lupus model was induced following JOVE protocol. Briefly, 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells from bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis after 2 weeks. Single cell suspensions of splenocytes from Bm12-induced lupus T-bet reporter mice were subjected to red blood cell lysis and filtered through 70 um nylon mesh.Cells were stained for surface markers under instructions. DP and DN B cells were collected through flow cytometry.","Sequencing - Illumina HiSeq X Ten","Nucleic Acid Extraction - Briefly, 50,000 live cells were freshly isolated and resuspended in lysis buffer (10 mM Tris·Cl,（pH 7.4）,10 mM NaCl，3 mM MgCl2，0.1% (v/v) Igepal CA-630) and pelleted. The cell pellet was resuspended in transposase reaction mix (5×TTBL 10μl, TTE Mix V50 5μl, ddH2O 35μl) and incubated at 37°C for 30 min."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["YANG JIANG","Nan Shen"],"additional_accession":[]},"is_claimable":false,"name":"ATAC-seq in naive B cells, CD11c+T-bet+ B cells,  CD11c-T-bet- B cells from Bm12-induced lupus T-bet reporter mice","description":"To test the chromatin accessibility of different immune cell subsets, we isolated naive B cells CD11c+T-bet+ B cells,  CD11c-T-bet- B cells by flow cytometry from Bm12-induced lupus T-bet reporter mice and performed ATAC assay.","dates":{"release":"2025-05-16T00:00:00Z","modification":"2023-05-16T15:29:45.616Z","creation":"2023-05-16T15:29:45.616Z"},"accession":"E-MTAB-12903","cross_references":{"ENA":["ERP147273"],"EFO":["EFO_0002944","EFO_0007045","EFO_0004170","EFO_0005518","EFO_0004184"]}}