biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsYANG JIANGIllumina NovaSeq 6000ATAC-seqMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-12903To test the chromatin accessibility of different immune cell subsets, we isolated naive B cells CD11c+T-bet+ B cells, CD11c-T-bet- B cells by flow cytometry from Bm12-induced lupus T-bet reporter mice and performed ATAC assay.biostudies-arrayexpressLibrary Construction - Fragmented DNA was purified using VAHTS DNA Clean Beads and library was generated by PCR.Sample Collection - bm12-cGVHD lupus model was induced following JOVE protocol. Briefly, 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells from bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis after 2 weeks. Single cell suspensions of splenocytes from Bm12-induced lupus T-bet reporter mice were subjected to red blood cell lysis and filtered through 70 um nylon mesh.Cells were stained for surface markers under instructions. DP and DN B cells were collected through flow cytometry.Sequencing - Illumina HiSeq X TenNucleic Acid Extraction - Briefly, 50,000 live cells were freshly isolated and resuspended in lysis buffer (10 mM Tris·Cl,（pH 7.4）,10 mM NaCl，3 mM MgCl2，0.1% (v/v) Igepal CA-630) and pelleted. The cell pellet was resuspended in transposase reaction mix (5×TTBL 10μl, TTE Mix V50 5μl, ddH2O 35μl) and incubated at 37°C for 30 min.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesYANG JIANGNan ShenfalseATAC-seq in naive B cells, CD11c+T-bet+ B cells, CD11c-T-bet- B cells from Bm12-induced lupus T-bet reporter miceTo test the chromatin accessibility of different immune cell subsets, we isolated naive B cells CD11c+T-bet+ B cells, CD11c-T-bet- B cells by flow cytometry from Bm12-induced lupus T-bet reporter mice and performed ATAC assay.2025-05-16T00:00:00Z2023-05-16T15:29:45.616Z2023-05-16T15:29:45.616ZE-MTAB-12903ERP147273EFO_0002944EFO_0007045EFO_0004170EFO_0005518EFO_0004184