{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["YANG JIANG"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["CUT&RUN"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-12904"],"description":["To clarify the regulation of Zeb2 and T-bet in ABCs, we used flow cytometry to sort 50 million mouse age-associated B cells and perform Cut&RUN assay."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - bm12-cGVHD lupus model was induced following JOVE protocol (10.3791/53319). Briefly, 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells from bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis after 2 weeks. Single cell suspensions of splenocytes from Bm12-induced lupus T-bet reporter mice were subjected to red blood cell lysis and filtered through 70 um nylon mesh. Cells were stained for surface markers under instructions. age-associated B cells were collected through flow cytometry.","Library Construction - CUT & RUN libraries were generated following instructions of the manufacturer’s protocol (CST, DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN),##56795)","Sequencing - Illumina HiSeq X Ten","Nucleic Acid Extraction - 0.5 million B cells were incubated with CoA-coated magnetic beads, and then primary antibodies (anti- Zeb2, anti-T-bet, anti-IgG)were added to incubate for 1 hour at room temperature. After incubation, secondary antibodies and pG-Tn5 adapter complex were added. Then,tagmentation took place for 1 hour, DNAs were extracted and purified, and PCR amplified into a final CUT&RUN library"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["YANG JIANG","Nan Shen"],"additional_accession":[]},"is_claimable":false,"name":"CUT&RUN for Zeb2, T-bet and IgG control in live mouse age-associated B cells","description":"To clarify the regulation of Zeb2 and T-bet in ABCs, we used flow cytometry to sort 50 million mouse age-associated B cells and perform Cut&RUN assay.","dates":{"release":"2025-05-12T00:00:00Z","modification":"2023-05-12T09:38:28.908Z","creation":"2023-05-12T09:38:28.908Z"},"accession":"E-MTAB-12904","cross_references":{"ENA":["ERP147172"],"EFO":["EFO_0002944","EFO_0009973","EFO_0004170","EFO_0005518","EFO_0004184"]}}