{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Yang Jiang"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["ATAC-seq"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-12905"],"description":["To test the differences in chromatin accessibility of spleen B cells between Zeb2-CKO mice and WT mice, we isolated naive B cells from B cell-specific Zeb2-CKO and WT mice and cultured them in ABC-inducing culture for 3 days and performed ATAC assay."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Splenic B cells from B-cell specific Zeb2-CKO mice and WT mice were purified by negative selection and were induced in the presence of R848, anti-CD40,anti-IgM F(ab’)2 , IL-21, IFN-γ for 3 days, then, cells were collected.","Library Construction - Fragmented DNA was purified using VAHTS DNA Clean Beads and library was generated by PCR.","Sequencing - Illumina HiSeq X Ten","Nucleic Acid Extraction - Briefly, 50,000 live cells were freshly isolated and resuspended in lysis buffer (10 mM Tris·Cl,（pH 7.4）,10 mM NaCl，3 mM MgCl2，0.1% (v/v) Igepal CA-630) and pelleted. The cell pellet was resuspended in transposase reaction mix (5×TTBL 10μl, TTE Mix V50 5μl, ddH2O 35μl) and incubated at 37°C for 30 min."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Yang Jiang","Nan Shen"],"additional_accession":[]},"is_claimable":false,"name":"ATAC-seq of spleen B cells from B cell-specific Zeb2-CKO and WT mice","description":"To test the differences in chromatin accessibility of spleen B cells between Zeb2-CKO mice and WT mice, we isolated naive B cells from B cell-specific Zeb2-CKO and WT mice and cultured them in ABC-inducing culture for 3 days and performed ATAC assay.","dates":{"release":"2025-05-12T00:00:00Z","modification":"2023-05-12T09:02:06.291Z","creation":"2023-05-12T09:02:06.291Z"},"accession":"E-MTAB-12905","cross_references":{"ENA":["ERP147168"],"EFO":["EFO_0002944","EFO_0007045","EFO_0004170","EFO_0005518","EFO_0004184"]}}