biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsYang JiangIllumina NovaSeq 6000ATAC-seqMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-12905To test the differences in chromatin accessibility of spleen B cells between Zeb2-CKO mice and WT mice, we isolated naive B cells from B cell-specific Zeb2-CKO and WT mice and cultured them in ABC-inducing culture for 3 days and performed ATAC assay.biostudies-arrayexpressSample Collection - Splenic B cells from B-cell specific Zeb2-CKO mice and WT mice were purified by negative selection and were induced in the presence of R848, anti-CD40,anti-IgM F(ab’)2 , IL-21, IFN-γ for 3 days, then, cells were collected.Library Construction - Fragmented DNA was purified using VAHTS DNA Clean Beads and library was generated by PCR.Sequencing - Illumina HiSeq X TenNucleic Acid Extraction - Briefly, 50,000 live cells were freshly isolated and resuspended in lysis buffer (10 mM Tris·Cl,（pH 7.4）,10 mM NaCl，3 mM MgCl2，0.1% (v/v) Igepal CA-630) and pelleted. The cell pellet was resuspended in transposase reaction mix (5×TTBL 10μl, TTE Mix V50 5μl, ddH2O 35μl) and incubated at 37°C for 30 min.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesYang JiangNan ShenfalseATAC-seq of spleen B cells from B cell-specific Zeb2-CKO and WT miceTo test the differences in chromatin accessibility of spleen B cells between Zeb2-CKO mice and WT mice, we isolated naive B cells from B cell-specific Zeb2-CKO and WT mice and cultured them in ABC-inducing culture for 3 days and performed ATAC assay.2025-05-12T00:00:00Z2023-05-12T09:02:06.291Z2023-05-12T09:02:06.291ZE-MTAB-12905ERP147168EFO_0002944EFO_0007045EFO_0004170EFO_0005518EFO_0004184