biostudies-arrayexpressSaccharomyces cerevisiaehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-12992We have performed paired-end RNA sequencing on S. cerevisiae (BY4741). Lanosterol (LST) was predicted as a potential modulator of the Ste2 (GPCR)-mediated pheromone-induced cell death using Gcoupler. The cells were grown at 30°C at 200 r.p.m. in YPD medium in biological duplicates in the presence or absence of lanosterol (LST) at a final concentration of 1 μM. Post-metabolite treatment, the cells were either treated or untreated with the α-factor at a final concentration of 30 μM for 2 hours at desired growth conditions. Post these steps, the cells were harvested, and RNA was isolated, and sequenced.biostudies-arrayexpressNucleic Acid Extraction - RNA isolation was done using TRIzol Reagent. The sample was homogenized with TRIzol Reagent, followed by addition of chloroform. The solution was allowed to separate into different layers, and the upper aqueous layer was transferred to isopropanol, in the presence of glycogen for precipitation. The precipitated RNA was washed with ethanol, and resuspended in nuclease-free water. DNAse treatment was done to remove DNA contamination. The pure RNA was again precipitated with ethanol, washed and resuspended in nuclease free water for downstream processing.Sequencing - Illumina Platform based RNA sequencing was performedSample Collection - The yeast culture was treated and then centrifuged.Library Construction - the nucleic acid library was successfully preparedGrowth Protocol - Yeast cells (BY4741) were grown at 30°C at 200 r.p.m. in YPD medium.Sample Treatment - The cells were grown in the presence or absence of lanosterol (LST) at a final concentration of 1 μM. Post-metabolite loading, the cells were either treated or untreated with the α-factor at a final concentration of 30 μM for 2 hours.MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNASaccharomyces cerevisiaeERP147620Gaurav AhujafalseRNA-seq of S. cerevisiae (BY4741) treated with Lanosterol against untreated controls in the presence and absence of alpha-factorWe have performed paired-end RNA sequencing on S. cerevisiae (BY4741). Lanosterol (LST) was predicted as a potential modulator of the Ste2 (GPCR)-mediated pheromone-induced cell death using Gcoupler. The cells were grown at 30°C at 200 r.p.m. in YPD medium in biological duplicates in the presence or absence of lanosterol (LST) at a final concentration of 1 μM. Post-metabolite treatment, the cells were either treated or untreated with the α-factor at a final concentration of 30 μM for 2 hours at desired growth conditions. Post these steps, the cells were harvested, and RNA was isolated, and sequenced.2025-08-01T00:00:00Z2024-12-04T08:40:27.164Z2023-05-23T15:19:25.016ZE-MTAB-12992ERP147620EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184EFO_0003969