biostudies-arrayexpressPete DayanandaHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13038The uploaded data has been generated from the analysis of nasal scrape samples from two clinical studies registered on ClinicalTrials.gov with identifier ID NCT02755948 and NCT04204993. Primary objectives were to establish the human infection challenge model using a novel H3N2 influenza A virus (IAV, influenza A/Belgium/4217/15) at Imperial College London and to test the hypothesis that influenza lead to quantitative and functional differences in innate and T-cell responses between infected, uninfected, symptomatic and asymptomatic participants. The data is to remain private until the publishing of the relative results.biostudies-arrayexpressSample Collection - Nasal curettage samples were collected at baseline, Days 1, 2, 3, 7, 10 and 14 post-inoculation and preserved in TRIzol. These were stored at -80°C until processing.Nucleic Acid Extraction - Total RNA was extracted from nasal curettage samples using Qiagen RNeasy Mini kit (Qiagen, Hilden, Germany).Sequencing - The sequencing libraries were clustered on flowcells, targeting 30 million paired-end reads persample. After clustering, the flowcells were loaded on the Illumina HiSeq4000. The samples were sequenced using a 2x150bp Paired End configuration. . Image analysis and base calling were conducted by theControl software. Raw sequence data (.bcl files) generated the sequencer were converted into fastq files and de-multiplexed using Illumina's bcl2fastq 2.17 software. One mismatch was allowed for index sequence identification.Library Construction - Strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA). The enriched RNAs were fragmented for 8 minutes at 94 °C. The first strand and second strand cDNA were subsequently synthesized. The second strand of cDNA was marked by incorporating dUTP during the synthesis. cDNA fragments were adenylated at 3’ends, and indexed adapter was ligated to cDNA fragments. Limited cycle PCR was used for library enrichment.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Sequencing reads were mapped to the reference transcriptome (Ensembl Human GRCh38.p13) using STAR (version 2.7.10a).Data Transformation - Data was normalised in DESEq2 (version 1.36) using the estimateSizeFactors and counts function.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 4000RNA-seq of coding RNAHomo sapiensLoukas PapargyrisPete DayanandafalseRNA-Seq of healthy adult volunteers following experimental infection with Influenza A/Belgium/4217/2015 (H3N2)The uploaded data has been generated from the analysis of nasal scrape samples from two clinical studies registered on ClinicalTrials.gov with identifier ID NCT02755948 and NCT04204993. Primary objectives were to establish the human infection challenge model using a novel H3N2 influenza A virus (IAV, influenza A/Belgium/4217/15) at Imperial College London and to test the hypothesis that influenza lead to quantitative and functional differences in innate and T-cell responses between infected, uninfected, symptomatic and asymptomatic participants. The data is to remain private until the publishing of the relative results.2026-07-01T00:00:00Z2026-07-01T20:51:55.14Z2023-06-08T07:45:25.756ZE-MTAB-13038ERP148001EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184