{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Myrsini Kaforou"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13041"],"description":["The Influenza H3N2 human challenge model has been described in detail previously5. Briefly, 20 healthy volunteers were inoculated intranasally with a standardized dose of Influenza A virus (A/Belgium/4217/2015(H3N2)) under quarantine conditions. From 24 hours after inoculation, virus was quantified by PCR in nasal lavage samples obtained at 12 hourly intervals. Participants were ascertained to have replicative viral infection if found to have consecutive positive PCR tests at least 24 hours after challenge. Blood samples for RNA sequencing were collected into PAXgene tubes before virus challenge and days 1, 2, 3, 7, 10, 14, and 28 after challenge. These were stored at -80°C until processing"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs)","Sample Collection - The samples were collected in a PAX gene tube and stored in -80","Nucleic Acid Extraction - The samples were extracted using the Qiagen automatic extraction process","Sequencing - sequenced on the Illumina NovaSeq 6000 platform using the NovaSeq 6000 S2 200 cycles Flowcell (Illumina), with a target of 40 million paired-end reads per sample."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - The data was normalised and transformed using DESeq2 in R software"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["R","Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["Claire Broderick","Myrsini Kaforou","Christopher Chiu"],"additional_accession":[]},"is_claimable":false,"name":"Whole blood RNA-Sequencing from an Influenza H3N2 human challenge model","description":"The Influenza H3N2 human challenge model has been described in detail previously5. Briefly, 20 healthy volunteers were inoculated intranasally with a standardized dose of Influenza A virus (A/Belgium/4217/2015(H3N2)) under quarantine conditions. From 24 hours after inoculation, virus was quantified by PCR in nasal lavage samples obtained at 12 hourly intervals. Participants were ascertained to have replicative viral infection if found to have consecutive positive PCR tests at least 24 hours after challenge. Blood samples for RNA sequencing were collected into PAXgene tubes before virus challenge and days 1, 2, 3, 7, 10, 14, and 28 after challenge. These were stored at -80°C until processing","dates":{"release":"2025-05-26T00:00:00Z","modification":"2023-05-30T12:00:51.951Z","creation":"2023-05-30T12:00:51.951Z"},"accession":"E-MTAB-13041","cross_references":{"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}