biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsElena Brunnertranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13167Primary human prostate fibroblast cultures were generated via an explant culture method from 4mm biopsies taken from radical prostatectomy samples of treatment-naive prostate cancer patients. The samples include partially patient-matched fibroblast cultures from benign- as well as cancer-associated regions.biostudies-arrayexpressSample Collection - Primary human prostate fibroblasts cultures were generated via an explant culture method from 4mm biopsies taken from radical prostatectomy samples. Cell pellets were taken from fibroblasts cultured undere routine conditions in fibroblast culture medium (DMEM containing 1000 g/L glucose supplemented with 10% FBS (PAN-Biotech GmbH), 2% GlutaMAXTM and 1% penicillin/streptomycin).Scaning - One microgram total RNA was hybridized to whole genome BeadChip® Sentrix arrays (HumanHT-12v4) at the German Cancer Genome Research Centre, Heidelberg, Germany). The procedure was performed as described in the following article: Rattay, K., Derbinski, J., Hofmann, T. G., & Kyewski, B. (2015). Genome-wide gene expression profiling of homeodomain-interacting protein kinase 2 deficient medullary thymic epithelial cells. Genomics data, 6, 48–50. https://doi.org/10.1016/j.gdata.2015.08.004Nucleic Acid Extraction - RNA was isolated using RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol.Labeling - One microgram total RNA was hybridized to whole genome BeadChip® Sentrix arrays (HumanHT-12v4) at the German Cancer Genome Research Centre, Heidelberg, Germany). The procedure was performed as described in the following article: Rattay, K., Derbinski, J., Hofmann, T. G., & Kyewski, B. (2015). Genome-wide gene expression profiling of homeodomain-interacting protein kinase 2 deficient medullary thymic epithelial cells. Genomics data, 6, 48–50. https://doi.org/10.1016/j.gdata.2015.08.004Hybridization - One microgram total RNA was hybridized to whole genome BeadChip® Sentrix arrays (HumanHT-12v4) at the German Cancer Genome Research Centre, Heidelberg, Germany). The procedure was performed as described in the following article: Rattay, K., Derbinski, J., Hofmann, T. G., & Kyewski, B. (2015). Genome-wide gene expression profiling of homeodomain-interacting protein kinase 2 deficient medullary thymic epithelial cells. Genomics data, 6, 48–50. https://doi.org/10.1016/j.gdata.2015.08.004MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsElena BrunnerNatalie SampsonData Transformation - One microgram total RNA was hybridized to whole genome BeadChip® Sentrix arrays (HumanHT-12v4) at the German Cancer Genome Research Centre, Heidelberg, Germany). The procedure was performed as described in the following article: Rattay, K., Derbinski, J., Hofmann, T. G., & Kyewski, B. (2015). Genome-wide gene expression profiling of homeodomain-interacting protein kinase 2 deficient medullary thymic epithelial cells. Genomics data, 6, 48–50. https://doi.org/10.1016/j.gdata.2015.08.004falseTranscription profiling of primary human prostate cancer-associated fibroblastsPrimary human prostate fibroblast cultures were generated via an explant culture method from 4mm biopsies taken from radical prostatectomy samples of treatment-naive prostate cancer patients. The samples include partially patient-matched fibroblast cultures from benign- as well as cancer-associated regions.2025-09-02T00:00:00Z2025-09-03T00:01:19.934Z2023-07-24T13:39:33.661ZE-MTAB-13167EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815