biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsBetty Ying-Wen ChungIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13212To identify the EGR1 dependence of the macrophage transcriptional response to Salmonella Typhimurium infection, RNA was harvested from CRISPR Egr1 knockout or CRISPR negative control (WT) immortalised bone marrow derived macrophages throughout a 4 hour infection.biostudies-arrayexpressLibrary Construction - Libraries were generated with the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD according to manufacturer's instructionsNucleic Acid Extraction - RNA was extracted from cultured cells at the indicated time point by Trizol purification according to manufacturer's instructionsSequencing - Sequencing and demultiplexing was performed by NovogeneSample Collection - A clonal population of immortalised bone marrow derrived macrophages following CRISPR KO of Egr1 or CRISPR against no target (i.e. WT) were infected with Salmonella enterica sv. Typhimurium (strain SL1344) at a multiplicity of infection of 10 or mock-infected. Gentamicin was added to cultures at 15 min post infection to kill extracellular bacteria.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesBetty Ying-Wen ChungfalseQuantSeq of EGR1 KO or WT immortalised bone marrow derived macrophages during Salmonella Typhimurium infectionTo identify the EGR1 dependence of the macrophage transcriptional response to Salmonella Typhimurium infection, RNA was harvested from CRISPR Egr1 knockout or CRISPR negative control (WT) immortalised bone marrow derived macrophages throughout a 4 hour infection.2025-08-29T00:00:00Z2025-08-30T00:01:13.888Z2023-08-04T09:53:50.914ZE-MTAB-13212ERP149959EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184