biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsRajendra LabalaIllumina HiSeq 2500SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, Austria)Trizol methodRNA-seq of total RNADanio rerioDanio reriohttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13218Artificial light at night (ALAN) alters zebrafish physiology. The present study performed on ALAN-induced (normal Light-Dark, LD; continuous light for one week, LLW; one month, LLM; and one year, LLY) ovarian transcriptome to investigate the effects it causes to the organism. The results displayed a desynchronisation of various genes and pathways leading to the initiation of several diseases, for the first time in zebrafish.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated from the sample using Trizol (Invitrogen) method for RNA extraction.Sample Collection - The adult female zebrafish (identical in age, body weight, and gonadal status) were randomly divided into four experimental groups in different photic conditions: Normal: LD = 12H L:12H D, Treated: (i) continuous light for one week, LLW (7 days), (ii) for one month, LLM (30 days), and (iii) for one year, LLY (365 days). All the samplings were performed at same time (12.00 H) The ovary was removed by dissecting the body cavity respectively. Sample for whole transcriptome sequencing was stored in RNAlater (Life Technology, USA)], and at -80°C.Library Construction - SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, Austria) was used as per the protocol to generate the libraries.Sequencing - 2 × 150 PE sequencingOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesRajendra LabalafalseRNA-Seq of zebrafish ovary treated with artificial light at night (ALAN) against a normal light dark sampleArtificial light at night (ALAN) alters zebrafish physiology. The present study performed on ALAN-induced (normal Light-Dark, LD; continuous light for one week, LLW; one month, LLM; and one year, LLY) ovarian transcriptome to investigate the effects it causes to the organism. The results displayed a desynchronisation of various genes and pathways leading to the initiation of several diseases, for the first time in zebrafish.2025-06-30T00:00:00Z2023-08-29T12:38:34.717Z2023-08-29T12:38:34.717ZE-MTAB-13218ERP150605EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184