{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Vincent Gabriel"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-1323"],"description":["Full thickness and deep partial thickness burn injuries heal by scarring.  There are several mechanisms thought to be essential for the development of burn scars, but a challenge to studying the skin response to burn injury is that there are few animal models of burn scarring that are either clinically similar to human burn scars or are practical for most investigators to use.  The purpose of this study was to examine the changes in RNA expression in human skin to burn injury.  This was done by comparing pre-injury tissue from otherwise healthy adults undergoing aesthetic scarification created by branding with a hot metal object to serial samples of untreated wounds in the same subjects."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - Subjects were recruited by self-identifying to the investigators when they presented to a body modification studio for aesthetic branding.  Punch biopsies were taken from normal skin, burn and scars.  The biopsies were placed immediately in to RNAlater and stored at 4C prior to RNA isolation.","Labeling - Labelling was performed according to the standard protocol used by The University of Texas Southwestern Medical Centre at Dallas as described in detail here:  http://microarray.swmed.edu/protocols/protocol/illumina-gene-expression-probe-labeling/","Hybridization - Standard Illumina protocol using Whole Genome Gene Expression Direct Hybridization  http://www.illumina.com/applications/detail/gene_expression_analysis/whole_genome_gene_expression.ilmn","Nucleic Acid Extraction - Samples were treated with Dnase I and extraction was pefromed using the QIAGEN Rneasy kit as described by the manufacturer.  http://www.qiagen.com  Quality control was performed with an Aglient Bioanalyser.  http://www.home.agilent.com"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Image Adquisition - Standard Illumina scanning protocols using Bead Array Reader were used http://support.illumina.com/array/array_instruments/beadarray_reader.ilmn . Please note that PROBE_ID ILMN_2038777 appears twice in each of the raw data files in this experiment, for yet unknown reasons."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"study_type":["transcription profiling by array"],"species":["Homo sapiens"],"pubmed_title":["RNA expression in untreated human burn wounds"],"pubmed_authors":["Vincent Gabriel"],"additional_accession":[]},"is_claimable":false,"name":"Human Scarring Project","description":"Full thickness and deep partial thickness burn injuries heal by scarring.  There are several mechanisms thought to be essential for the development of burn scars, but a challenge to studying the skin response to burn injury is that there are few animal models of burn scarring that are either clinically similar to human burn scars or are practical for most investigators to use.  The purpose of this study was to examine the changes in RNA expression in human skin to burn injury.  This was done by comparing pre-injury tissue from otherwise healthy adults undergoing aesthetic scarification created by branding with a hot metal object to serial samples of untreated wounds in the same subjects.","dates":{"release":"2014-01-22T00:00:00Z","modification":"2022-03-14T15:32:23.052Z","creation":"2022-03-14T15:32:23.052Z"},"accession":"E-MTAB-1323","cross_references":{"EFO":["EFO_0002768"]}}