{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13343"],"description":["Analysis of the global variability of gene expression between the Eif6 WT control genotype and the Eif6 S235A mutated MEF lines. To analyze the effects over time of the lack of Eif6 phosphorylation, we transduced MEFs with tamoxifen-inducible Cre, so that treatment leads to excision of wild-type exons and the generation of cells bearing the S235A mutation. RNA-seq analyses were performed on samples collected at the time of tamoxifen addition (T0), 48 hours (T1), and 72 hours (T2) after the treatment."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Sample processing, quality controls, library preparation, sequencing and alignment have been performed by IGATech (Udine, Via J.Linussio 51 Z.I.U., 33100, Italy).","Nucleic Acid Extraction - Sample processing, quality controls, library preparation, sequencing and alignment have been performed by IGATech (Udine, Via J.Linussio 51 Z.I.U., 33100, Italy).","Sequencing - single-end 75 bp mode on the NextSeq 500 platform","Library Construction - TruSeq Stranded mRNA kit, fr-firststrand protocol"],"figure_sub":["MINSEQE Score","Assays and Data","Processed Data","organisation","MAGE-TAB Files"],"data_protocol":["Data Transformation - standard RNA-seq pipeline"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"additional_accession":["ERP151076"],"pubmed_authors":["Ivan Ferrari"]},"is_claimable":false,"name":"RNA-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)","description":"Analysis of the global variability of gene expression between the Eif6 WT control genotype and the Eif6 S235A mutated MEF lines. To analyze the effects over time of the lack of Eif6 phosphorylation, we transduced MEFs with tamoxifen-inducible Cre, so that treatment leads to excision of wild-type exons and the generation of cells bearing the S235A mutation. RNA-seq analyses were performed on samples collected at the time of tamoxifen addition (T0), 48 hours (T1), and 72 hours (T2) after the treatment.","dates":{"release":"2026-02-10T00:00:00Z","modification":"2026-05-27T18:06:19.949Z","creation":"2023-09-11T17:29:06.335Z"},"accession":"E-MTAB-13343","cross_references":{"ENA":["ERP151076"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}