{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13350"],"description":["UK-5099 is a pharmacological inhibitor of MPC1, a protein that transports pyruvate into mitochondria. Human hair follicles were treated with vehicle, and 10 µM and 40 µM UK-5099 for 48h ex vivo. RNASeq was performed to determine whether MPC1 inhibition induces transcriptional changes in the hair follicle."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.","Growth Protocol - Human hair follicles were cultured in an ex vivo organ culture model as described previously (Purba et al., 2019).","Nucleic Acid Extraction - Total RNA was extracted using RNeasy Micro Kit (Qiagen #74004), according to the manufacturer’s protocol. Total RNA was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF). Quality and integrity of the RNA samples were assessed using a 2200 TapeStation (Agilent Technologies).","Sequencing - Sequenced paired end 76bp on HISEQ4000 at the Genomic Technologies Core Facility University of Manchester.","Sample Treatment - Human hair follicles were subject to treatment with UK-5099 (10 and 40 µM Tocris #4186) for 48 h before isolation, embedding in frozen medium and freezing in liquid nitrogen or storage in RNA later.","Sample Collection - Human hair follicle samples were obtained from consenting patients from the Crown Clinic, Manchester and were handled and stores in line with HTA regulations and ethical approval."],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina HiSeq 4000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_title":["CDK4/6 inhibition mitigates stem cell damage in a novel model for taxane-induced alopecia."],"additional_accession":["ERP151207"],"pubmed_authors":["Talveen Purba","Rachel Scholey","Purba TS, Ng'andu K, Brunken L, Smart E, Mitchell E, Hassan N, O'Brien A, Mellor C, Jackson J, Shahmalak A, Paus R."]},"is_claimable":false,"name":"Mitochondrial Pyruvate Carrier Inhibition Activates the Integrated Stress Response in Human Hair Follicles - RNAseq","description":"UK-5099 is a pharmacological inhibitor of MPC1, a protein that transports pyruvate into mitochondria. Human hair follicles were treated with vehicle, and 10 µM and 40 µM UK-5099 for 48h ex vivo. RNASeq was performed to determine whether MPC1 inhibition induces transcriptional changes in the hair follicle.","dates":{"release":"2025-07-16T00:00:00Z","modification":"2025-07-16T09:29:03.342Z","creation":"2023-09-15T12:39:27.743Z"},"accession":"E-MTAB-13350","cross_references":{"ENA":["ERP151207"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003738","EFO_0003969","EFO_0004184"]}}