biostudies-arrayexpressHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13350UK-5099 is a pharmacological inhibitor of MPC1, a protein that transports pyruvate into mitochondria. Human hair follicles were treated with vehicle, and 10 µM and 40 µM UK-5099 for 48h ex vivo. RNASeq was performed to determine whether MPC1 inhibition induces transcriptional changes in the hair follicle.biostudies-arrayexpressLibrary Construction - Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.Growth Protocol - Human hair follicles were cultured in an ex vivo organ culture model as described previously (Purba et al., 2019).Nucleic Acid Extraction - Total RNA was extracted using RNeasy Micro Kit (Qiagen #74004), according to the manufacturer’s protocol. Total RNA was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF). Quality and integrity of the RNA samples were assessed using a 2200 TapeStation (Agilent Technologies).Sequencing - Sequenced paired end 76bp on HISEQ4000 at the Genomic Technologies Core Facility University of Manchester.Sample Treatment - Human hair follicles were subject to treatment with UK-5099 (10 and 40 µM Tocris #4186) for 48 h before isolation, embedding in frozen medium and freezing in liquid nitrogen or storage in RNA later.Sample Collection - Human hair follicle samples were obtained from consenting patients from the Crown Clinic, Manchester and were handled and stores in line with HTA regulations and ethical approval.MINSEQE ScoreAssays and DataorganisationMAGE-TAB FilesUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 4000RNA-seq of coding RNAHomo sapiensCDK4/6 inhibition mitigates stem cell damage in a novel model for taxane-induced alopecia.ERP151207Talveen PurbaRachel ScholeyPurba TS, Ng'andu K, Brunken L, Smart E, Mitchell E, Hassan N, O'Brien A, Mellor C, Jackson J, Shahmalak A, Paus R.falseMitochondrial Pyruvate Carrier Inhibition Activates the Integrated Stress Response in Human Hair Follicles - RNAseqUK-5099 is a pharmacological inhibitor of MPC1, a protein that transports pyruvate into mitochondria. Human hair follicles were treated with vehicle, and 10 µM and 40 µM UK-5099 for 48h ex vivo. RNASeq was performed to determine whether MPC1 inhibition induces transcriptional changes in the hair follicle.2025-07-16T00:00:00Z2025-07-16T09:29:03.342Z2023-09-15T12:39:27.743ZE-MTAB-13350ERP151207EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0003969EFO_0004184