{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Syed Murtuza Baker"],"instrument_platform":["NextSeq 500"],"study_type":["single nucleus RNA sequencing"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13453"],"description":["Congenital heart disease (CHD) is a major birth defect affecting ~1% of new born babies. The OFT carries blood away from the heart into the great arteries. Defects specifically affecting the outflow tract (OFT) of the heart represent a third of all CHD cases. In humans the formation and remodelling of the OFT is a relatively rapid process, occurring over a 3-week period (Carnegie Stage (CS)13 – CS22). The morphological changes underlying OFT formation are orchestrated by two main cell lineages, second heart field (SHF)-derived myocardium and endothelium, and neural crest cells (NCC). Here, we present comprehensive transcriptomic data on the developing OFT at two distinct timepoint (embryonic and fetal) and its adult derivatives, providing a large reference framework of OFT cell repertoires and their gene expression profiles. This submission has the Adult dataset of this work."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Human embryonic/fetal outflow tracts were collected after pregnancy termination. Adult human aortic valves were collected from hearts that were disqualified from transplant, FACS sorted for intact nuclei.","Nucleic Acid Extraction - Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdf","Library Construction - Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdf","Sequencing - Used Illumina NextSeq 500 protocol, detailed in nextseq-500-system-guide-15046563-06.pdf"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Nicoletta Bobola","Rotem Leshem","Syed Murtuza Baker"],"additional_accession":[]},"is_claimable":false,"name":"A cell atlas of the human outflow tract of the heart and its adult derivatives - Adult samples","description":"Congenital heart disease (CHD) is a major birth defect affecting ~1% of new born babies. The OFT carries blood away from the heart into the great arteries. Defects specifically affecting the outflow tract (OFT) of the heart represent a third of all CHD cases. In humans the formation and remodelling of the OFT is a relatively rapid process, occurring over a 3-week period (Carnegie Stage (CS)13 – CS22). The morphological changes underlying OFT formation are orchestrated by two main cell lineages, second heart field (SHF)-derived myocardium and endothelium, and neural crest cells (NCC). Here, we present comprehensive transcriptomic data on the developing OFT at two distinct timepoint (embryonic and fetal) and its adult derivatives, providing a large reference framework of OFT cell repertoires and their gene expression profiles. This submission has the Adult dataset of this work.","dates":{"release":"2025-06-09T00:00:00Z","modification":"2024-09-02T11:00:59.484Z","creation":"2023-10-20T14:43:48.863Z"},"accession":"E-MTAB-13453","cross_references":{"ENA":["ERP152688"],"Biostudies":["E-MTAB-13447"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009809","EFO_0005518","EFO_0004184"]}}