biostudies-arrayexpressSyed Murtuza BakerHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13461Congenital heart disease (CHD) is a major birth defect affecting ~1% of new born babies. The OFT carries blood away from the heart into the great arteries. Defects specifically affecting the outflow tract (OFT) of the heart represent a third of all CHD cases. In humans the formation and remodelling of the OFT is a relatively rapid process, occurring over a 3-week period (Carnegie Stage (CS)13 – CS22). The morphological changes underlying OFT formation are orchestrated by two main cell lineages, second heart field (SHF)-derived myocardium and endothelium, and neural crest cells (NCC). Here, we present comprehensive transcriptomic data on the developing OFT at two distinct timepoint (embryonic and fetal) and its adult derivatives, providing a large reference framework of OFT cell repertoires and their gene expression profiles. This submission has the Spatial Transcriptomics of this work.biostudies-arrayexpressLibrary Construction - Used 10X sample collection protocol, CG000239_VisiumSpatialGeneExpression_UserGuide_RevENucleic Acid Extraction - Used 10X sample collection protocol, detailed in CG000239_VisiumSpatialGeneExpression_UserGuide_RevESample Collection - Human fetal heart was collected after pregnancy termination and snap frozen, then embedded in OCT according to 10x protocol for Visium, and Cryosectioned at 10 micrometerSequencing - Used Illumina NextSeq 500 protocol, detailed in nextseq-500-system-guide-15046563-06.pdfOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - We used Cloupe image alignemnt program to align the images.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 500spatial transcriptomics by high-throughput sequencingHomo sapiensNicoletta BobolaRotem LeshemSyed Murtuza BakerfalseA cell atlas of the human outflow tract of the heart and its adult derivatives - 10X visium samplesCongenital heart disease (CHD) is a major birth defect affecting ~1% of new born babies. The OFT carries blood away from the heart into the great arteries. Defects specifically affecting the outflow tract (OFT) of the heart represent a third of all CHD cases. In humans the formation and remodelling of the OFT is a relatively rapid process, occurring over a 3-week period (Carnegie Stage (CS)13 – CS22). The morphological changes underlying OFT formation are orchestrated by two main cell lineages, second heart field (SHF)-derived myocardium and endothelium, and neural crest cells (NCC). Here, we present comprehensive transcriptomic data on the developing OFT at two distinct timepoint (embryonic and fetal) and its adult derivatives, providing a large reference framework of OFT cell repertoires and their gene expression profiles. This submission has the Spatial Transcriptomics of this work.2025-06-09T00:00:00Z2024-09-02T11:01:10.626Z2023-10-23T19:32:29.604ZE-MTAB-13461ERP152726E-MTAB-13447E-MTAB-13453EFO_0002944EFO_0004170EFO_0030005EFO_0005518EFO_0003816EFO_0004184