{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13517"],"description":["Following observations that sex of wild animals is associated with the acidity of colonic mucin glycans, as determined via histology, RNA sequencing was undertaken to explore potential mechanisms driving these differences. Colonic tissue samples from 24 male mice trapped on the Isle of May in October 2019 were selected, encompassing a range of staining profiles, following Alcian Blue PAS staining of colonic tissue. Using IPA software Esr1 signalling was identified as the major upstream regulator of gene expression associated with varied mucin acidity."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Sequenced paired end 76bp on HISEQ4000 at the Genomic Technologies Core Facility University of Manchester","Nucleic Acid Extraction - Total RNA was extracted using standard precipitation protocols using chloroform and isopropanol and was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF).","Sample Collection - Following excision, proximal colonic tissue was stored in 1mL of phenol reagent, and immediately frozen with dry ice before being stored at -80˚ C","Library Construction - Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol"],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina HiSeq 4000"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"additional_accession":["ERP153138"],"pubmed_authors":["David Thornton","Rachel Scholey"]},"is_claimable":false,"name":"Sex drives colonic mucin sialylation in wild mice - RNAseq","description":"Following observations that sex of wild animals is associated with the acidity of colonic mucin glycans, as determined via histology, RNA sequencing was undertaken to explore potential mechanisms driving these differences. Colonic tissue samples from 24 male mice trapped on the Isle of May in October 2019 were selected, encompassing a range of staining profiles, following Alcian Blue PAS staining of colonic tissue. Using IPA software Esr1 signalling was identified as the major upstream regulator of gene expression associated with varied mucin acidity.","dates":{"release":"2025-06-02T00:00:00Z","modification":"2025-06-02T13:04:27.062Z","creation":"2023-11-02T16:38:52.013Z"},"accession":"E-MTAB-13517","cross_references":{"ENA":["ERP153138"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}