biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMeriç ATAMANNextSeq 500Ribo-seqMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13568We carried out ribosome footprinting on the Quadriceps femoris muscle of 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated. The treatments were started at the age of 15 months. The main goal of the study was to decipher the translation changes in wild-type aging and upon above mentioned anti-aging treatments. We further investigated non-canonical targets of translational control and identify novel translational units.biostudies-arrayexpressSample Collection - Mice were sacrificed using CO2 inhalation and muscle were extracted and snap freezed immediately. All procedures were performed in accordance with Swiss regulations for animal experimentation, and approved by the veterinary commission of the Canton Basel-StadtLibrary Construction - SMARTer smRNA-Seq Kit for Illumina, Takara BioNucleic Acid Extraction - Hot phenol extraction of RNASequencing - Illumina RTA Version: 2.11.3, Sequencer : GFB NextSeq 500 (R2D2), Run type: single read Number of cycles: 76 Kit : v2OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMeriç ATAMANfalseRibo-seq of quadriceps muscle tissues from 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated adult miceWe carried out ribosome footprinting on the Quadriceps femoris muscle of 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated. The treatments were started at the age of 15 months. The main goal of the study was to decipher the translation changes in wild-type aging and upon above mentioned anti-aging treatments. We further investigated non-canonical targets of translational control and identify novel translational units.2025-11-23T00:00:00Z2025-11-23T02:02:06.681Z2023-11-27T13:30:28.747ZE-MTAB-13568ERP155445EFO_0002944EFO_0004170EFO_0008891EFO_0005518EFO_0004184