{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Meriç ATAMAN"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of total RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13569"],"description":["We carried out RNA sequencing on the Quadriceps femoris muscle of 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated. The treatments were started at the age of 15 months. The main goal of the study was to decipher the transcriptional changes in wild-type aging and upon above mentioned anti-aging treatments as well as to estimate the translation efficiency using ribo-seq and RNA-seq data."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Mice were sacrificed using CO2 inhalation and muscle were extracted and snap freezed immediately. All procedures were performed in accordance with Swiss regulations for animal experimentation, and approved by the veterinary commission of the Canton Basel-Stadt. Treatment was with rapamycin at 4.2 milligram per kilogram per day","Nucleic Acid Extraction - Hot phenol extraction of RNA","Library Construction - SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian","Sequencing - Illumina RTA Version: 2.11.3, Sequencer : GFB NextSeq 500 (R2D2), Run type: single read Number of cycles: 76 Kit : v2"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Meriç ATAMAN"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of quadriceps muscle tissues from 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated adult mice","description":"We carried out RNA sequencing on the Quadriceps femoris muscle of 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated. The treatments were started at the age of 15 months. The main goal of the study was to decipher the transcriptional changes in wild-type aging and upon above mentioned anti-aging treatments as well as to estimate the translation efficiency using ribo-seq and RNA-seq data.","dates":{"release":"2025-11-23T00:00:00Z","modification":"2025-11-23T02:02:06.677Z","creation":"2023-11-27T13:32:19.267Z"},"accession":"E-MTAB-13569","cross_references":{"ENA":["ERP155531"],"Biostudies":["E-MTAB-13568"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0004184"]}}