{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Rajendra Labala"],"instrument_platform":["Agilent Bioanalyzer 2100 system (Agilent Technologies, California, USA)","Illumina TruSeq Stranded Total RNA kit, Biomek FxP (Beckman Coulter)","NextSeq 500"],"study_type":["RNA-seq of total RNA"],"organism":["Oryzias latipes"],"species":["Oryzias latipes"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13591"],"description":["The present study is trying to decipher the role of Pax6.1(5a) Isoform in Oryzias latipes. Thus, samples of wild type and Pax6.1-5a-/- knockout total RNA were subjected to bulk RNAseq."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA was isolated using TRIzolTM Reagent according to TRIzolTM Reagent User Guide. Quality and purity of isolated RNA were determined by 1% of agarose gels. RNA integrity was evaluated via Agilent Bioanalyzer 2100 system using Eukaryote Total RNA Nano and Eukaryote Total RNA Pico assays.","Sequencing - Sequencing was performed by NextSeq 500 system (Illumina). 4-plex pools were loaded on the Illumina sequencer NextSeq 500, sequenced uni-directionally, generating ~500 million single-end reads, each 75 bases long.","Library Construction - The libraries were prepared using the Illumina TruSeq Stranded Total RNA kit in the automation system Biomek FxP.","Sample Collection - WT and Pax6.1-5a-/- knockout embryos collected, incubated to develop into stage 24 and dechorionated."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Rajendra Labala"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq comparison of Pax6.1(5a) Isoform knockout and Oryzias latipes wild type","description":"The present study is trying to decipher the role of Pax6.1(5a) Isoform in Oryzias latipes. Thus, samples of wild type and Pax6.1-5a-/- knockout total RNA were subjected to bulk RNAseq.","dates":{"release":"2025-12-01T00:00:00Z","modification":"2025-12-01T02:01:45.379Z","creation":"2023-12-01T23:30:17.772Z"},"accession":"E-MTAB-13591","cross_references":{"ENA":["ERP155713"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0004184"]}}