biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsRajendra LabalaAgilent Bioanalyzer 2100 system (Agilent Technologies, California, USA)Illumina TruSeq Stranded Total RNA kit, Biomek FxP (Beckman Coulter)NextSeq 500RNA-seq of total RNAOryzias latipesOryzias latipeshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13591The present study is trying to decipher the role of Pax6.1(5a) Isoform in Oryzias latipes. Thus, samples of wild type and Pax6.1-5a-/- knockout total RNA were subjected to bulk RNAseq.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated using TRIzolTM Reagent according to TRIzolTM Reagent User Guide. Quality and purity of isolated RNA were determined by 1% of agarose gels. RNA integrity was evaluated via Agilent Bioanalyzer 2100 system using Eukaryote Total RNA Nano and Eukaryote Total RNA Pico assays.Sequencing - Sequencing was performed by NextSeq 500 system (Illumina). 4-plex pools were loaded on the Illumina sequencer NextSeq 500, sequenced uni-directionally, generating ~500 million single-end reads, each 75 bases long.Library Construction - The libraries were prepared using the Illumina TruSeq Stranded Total RNA kit in the automation system Biomek FxP.Sample Collection - WT and Pax6.1-5a-/- knockout embryos collected, incubated to develop into stage 24 and dechorionated.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesRajendra LabalafalseRNA-seq comparison of Pax6.1(5a) Isoform knockout and Oryzias latipes wild typeThe present study is trying to decipher the role of Pax6.1(5a) Isoform in Oryzias latipes. Thus, samples of wild type and Pax6.1-5a-/- knockout total RNA were subjected to bulk RNAseq.2025-12-01T00:00:00Z2025-12-01T02:01:45.379Z2023-12-01T23:30:17.772ZE-MTAB-13591ERP155713EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184