biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsChristine HorlowIllumina NovaSeq 6000genotyping by high throughput sequencingArabidopsis thalianaArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13630To allow identification of causal snp involved in eskimo1 dwarfism suppression (beem) after an ems treatment of esk1 mutant, plants with larger rosettes than those of esk1 mutant were selected and back-crossed esk1 mutant. In case of recessive mutation, the F1 progeny presented esk1 phenotype and werer selfpollinated. In case of dominant mutation, the F1 progeny were crossed with esk1 and the next generation produced 100% beem phenotype. All beem lines were submitted to 5 cycles of backcross(BC) with esk1 mutant and F2 segregation observation. In F2BC5, bulk of 10 main inflorescences of each phenotype (beem and esk1) were collected by beem line. DNA of these bulks were extracted and whole genomes were sequenced.biostudies-arrayexpressSequencing - NovaSeq 6000 SP 300cequimolar sample % Phi X =1.0%Sample Treatment - DNA concentration was too low. Wasking and new DNA conentration was performed with Microcon DNA Fast Flow.Sonication (~350pb)Growth Protocol - Plants were grown in individual pot and watered with nutrient solution in greenhouse.Library Construction - kit Kapa Hyper Prep PCR Free Dual Index 2x8basesNucleic Acid Extraction - DNA extraction with Nucleospin Plant II Maxi -Macherey-Nagel kitSample Collection - Collect of main inflorescences of 10 beem plants and of 10 esk1 plants from the same progeny at F2BC5 generation.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesNadia BESSOLTANEChristine HorlowfalseBEEM : Biomass enhancement under eskimo1 mutationTo allow identification of causal snp involved in eskimo1 dwarfism suppression (beem) after an ems treatment of esk1 mutant, plants with larger rosettes than those of esk1 mutant were selected and back-crossed esk1 mutant. In case of recessive mutation, the F1 progeny presented esk1 phenotype and werer selfpollinated. In case of dominant mutation, the F1 progeny were crossed with esk1 and the next generation produced 100% beem phenotype. All beem lines were submitted to 5 cycles of backcross(BC) with esk1 mutant and F2 segregation observation. In F2BC5, bulk of 10 main inflorescences of each phenotype (beem and esk1) were collected by beem line. DNA of these bulks were extracted and whole genomes were sequenced.2025-12-13T00:00:00Z2025-12-13T02:02:16.871Z2023-12-14T13:32:00.671ZE-MTAB-13630ERP156029EFO_0002944EFO_0004170EFO_0003789EFO_0002771EFO_0005518EFO_0004184EFO_0003969