{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":[null],"study_type":["transcription profiling by array"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13669"],"description":["CD9+ progenitors were sorted from the epididymal adipose tissue of of lean and obese animals, and their transcriptomic profiles were performed using Microarray."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen).","Labeling - Total RNA was amplified and labeled with biotin using Ovation Pico WTA System V2 (NuGEN) and Encore BiotinIL Module (NuGEN) kits according to the manufacturer’s protocol.","Sample Collection - Mouse epidydimal adipose tissue was harvested and digested in DMEM and collagenase A. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, the adipocyte fraction is discarded and the stroma vascular fraction passed through a 100 µm filter before staining.Cell suspensions were stained with appropriate antibodies for 30 min on ice.Cell sorting was performed on a BD FACSAria II™ cell sorter.","Hybridization - Standard Illumina hybridization protocol.","Scaning - Standard Illumina scanning protocol."],"additional_accession":["E-MTAB-13670"],"figure_sub":["MIAME Score","Raw Data","Assays and Data","organisation","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Dussaud sébastien"]},"is_claimable":false,"name":"Microarray of CD9+ progenitors in the epididymal adipose tissue of lean and obese animals (C3H background)","description":"CD9+ progenitors were sorted from the epididymal adipose tissue of of lean and obese animals, and their transcriptomic profiles were performed using Microarray.","dates":{"release":"2025-05-23T00:00:00Z","modification":"2026-05-30T15:55:21.699Z","creation":"2023-12-22T13:38:27.403Z"},"accession":"E-MTAB-13669","cross_references":{"Biostudies":["E-MTAB-13670"],"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0005518","EFO_0003815"]}}