biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicstranscription profiling by arrayMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13669CD9+ progenitors were sorted from the epididymal adipose tissue of of lean and obese animals, and their transcriptomic profiles were performed using Microarray.biostudies-arrayexpressNucleic Acid Extraction - RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen).Labeling - Total RNA was amplified and labeled with biotin using Ovation Pico WTA System V2 (NuGEN) and Encore BiotinIL Module (NuGEN) kits according to the manufacturer’s protocol.Sample Collection - Mouse epidydimal adipose tissue was harvested and digested in DMEM and collagenase A. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, the adipocyte fraction is discarded and the stroma vascular fraction passed through a 100 µm filter before staining.Cell suspensions were stained with appropriate antibodies for 30 min on ice.Cell sorting was performed on a BD FACSAria II™ cell sorter.Hybridization - Standard Illumina hybridization protocol.Scaning - Standard Illumina scanning protocol.E-MTAB-13670MIAME ScoreRaw DataAssays and DataorganisationMAGE-TAB FilesArray DesignsDussaud sébastienfalseMicroarray of CD9+ progenitors in the epididymal adipose tissue of lean and obese animals (C3H background)CD9+ progenitors were sorted from the epididymal adipose tissue of of lean and obese animals, and their transcriptomic profiles were performed using Microarray.2025-05-23T00:00:00Z2026-05-30T15:55:21.699Z2023-12-22T13:38:27.403ZE-MTAB-13669E-MTAB-13670EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003815