{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":[null],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13670"],"description":["The fibro-infammatory progenitors and mesothelial cells were sorted from the epididymal adipose tissue of high fat diet fed mice treated with vehicle or with Nintedanib+celecoxib, and their transcriptomic profiles were performed using RNAseq."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche).","Nucleic Acid Extraction - RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen).","Sequencing - RNA-Seq libraries were sequenced on an Illumina NovaSeq 6000.","Sample Collection - Mouse epidydimal adipose tissue was harvested and digested in DMEM and collagenase A. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, the adipocyte fraction is discarded and the stroma vascular fraction passed through a 100 µm filter before staining.Cell suspensions were stained with appropriate antibodies for 30 min on ice.Cell sorting was performed on a BD FACSAria II™ cell sorter."],"figure_sub":["MINSEQE Score","Assays and Data","organisation","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"additional_accession":["E-MTAB-13669"],"pubmed_authors":["Dussaud sébastien"]},"is_claimable":false,"name":"RNA-seq of fibro-infammatory progenitors and  mesothelial cells in the epididymal adipose tissue of high fat diet fed animal treated with Vehicle or Nintedanib+Celecoxib (C3H background)","description":"The fibro-infammatory progenitors and mesothelial cells were sorted from the epididymal adipose tissue of high fat diet fed mice treated with vehicle or with Nintedanib+celecoxib, and their transcriptomic profiles were performed using RNAseq.","dates":{"release":"2025-05-23T00:00:00Z","modification":"2026-06-05T21:15:29.111Z","creation":"2023-12-22T13:39:11.147Z"},"accession":"E-MTAB-13670","cross_references":{"ENA":["ERP156304"],"Biostudies":["E-MTAB-13669"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}