<HashMap><database>biostudies-arrayexpress</database><scores/><additional><submitter/><organism>Mus musculus</organism><full_dataset_link>https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13670</full_dataset_link><description>The fibro-infammatory progenitors and mesothelial cells were sorted from the epididymal adipose tissue of high fat diet fed mice treated with vehicle or with Nintedanib+celecoxib, and their transcriptomic profiles were performed using RNAseq.</description><repository>biostudies-arrayexpress</repository><sample_protocol>Library Construction - cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche).</sample_protocol><sample_protocol>Nucleic Acid Extraction - RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen).</sample_protocol><sample_protocol>Sequencing - RNA-Seq libraries were sequenced on an Illumina NovaSeq 6000.</sample_protocol><sample_protocol>Sample Collection - Mouse epidydimal adipose tissue was harvested and digested in DMEM and collagenase A. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, the adipocyte fraction is discarded and the stroma vascular fraction passed through a 100 µm filter before staining.Cell suspensions were stained with appropriate antibodies for 30 min on ice.Cell sorting was performed on a BD FACSAria II™ cell sorter.</sample_protocol><figure_sub>MINSEQE Score</figure_sub><figure_sub>Assays and Data</figure_sub><figure_sub>organisation</figure_sub><figure_sub>MAGE-TAB Files</figure_sub><omics_type>Metabolomics</omics_type><omics_type>Unknown</omics_type><omics_type>Transcriptomics</omics_type><omics_type>Genomics</omics_type><omics_type>Proteomics</omics_type><instrument_platform>Illumina NovaSeq 6000</instrument_platform><study_type>RNA-seq of coding RNA</study_type><species>Mus musculus</species><additional_accession>E-MTAB-13669</additional_accession><pubmed_authors>Dussaud sébastien</pubmed_authors></additional><is_claimable>false</is_claimable><name>RNA-seq of fibro-infammatory progenitors and  mesothelial cells in the epididymal adipose tissue of high fat diet fed animal treated with Vehicle or Nintedanib+Celecoxib (C3H background)</name><description>The fibro-infammatory progenitors and mesothelial cells were sorted from the epididymal adipose tissue of high fat diet fed mice treated with vehicle or with Nintedanib+celecoxib, and their transcriptomic profiles were performed using RNAseq.</description><dates><release>2025-05-23T00:00:00Z</release><modification>2026-06-05T21:15:29.111Z</modification><creation>2023-12-22T13:39:11.147Z</creation></dates><accession>E-MTAB-13670</accession><cross_references><ENA>ERP156304</ENA><Biostudies>E-MTAB-13669</Biostudies><EFO>EFO_0002944</EFO><EFO>EFO_0004170</EFO><EFO>EFO_0005518</EFO><EFO>EFO_0003738</EFO><EFO>EFO_0004184</EFO></cross_references></HashMap>