biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsHagit PhilipIllumina MiSeqRNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13684Transcriptome Analysis via RNA Sequencing in Treated Haploid Human Embryonic Stem Cells (hESCs) with Wild-Type (WT) or MLH1 or TP53 Knockout, and Multiple Colorectal Cancer Cell Lines Undergoing Treatment or Remaining Untreated with Various Inhibitors.biostudies-arrayexpressSample Collection - Wash cells with PBS x2. Trypsinize (TrypLE Select) the wells with the regular protocol. Spin down the cells after neutralizing the trypsin with a serum-containing medium. Count cells freeze pellet for RNA extractionSequencing - MiSeq IlluminaNucleic Acid Extraction - Qiagen RNeasyLibrary Construction - kitOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesHagit PhilipfalseTreatment Effects of BI2536, SR1848, ON1231320, and PaB on WT and MLH1 or TP53 Knockout Haploid Human Embryonic Stem Cells and Various Colorectal Cancer Cell LinesTranscriptome Analysis via RNA Sequencing in Treated Haploid Human Embryonic Stem Cells (hESCs) with Wild-Type (WT) or MLH1 or TP53 Knockout, and Multiple Colorectal Cancer Cell Lines Undergoing Treatment or Remaining Untreated with Various Inhibitors.2025-12-17T00:00:00Z2025-12-17T02:02:16.143Z2024-01-02T17:06:36.497ZE-MTAB-13684ERP156423E-MTAB-13685E-MTAB-13682E-MTAB-13683EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184