biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsCharles GirardotNextSeq 500ATAC-seqMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-13692To describe chromatin accessibility changes associated with differentiation of mouse presomitic mesoderm (PSM) cells into somites, ATAC sequencing (paired-end) was performed using non-cultured, wild-type PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions. Omni-ATAC protocol was used, and each condition includes six biological replicates.�biostudies-arrayexpressSequencing - paired-end sequencing on illumina NextSeq 500Library Construction - ATAC-seq library were prepared following the Omni-ATAC protocol (Corces et al., Nat Methods, 2017).Growth Protocol - Mice were crossed, and females were dissected 10 days after a detection of vaginal plug, and the PSM tissues isolated.Growth Protocol - PSM explants of E10.5 wild-type embryos were microdissected into tail bud, posterior PSM, anterior PSM, and somite regions by micro scalpel in cold PBS. Each tissue region was transferred into a micro well (ibidi, #80486) and mechanically dissociated to a cell suspension in 4.2 �l cold PBS. Finally, 0.7 �l and 3.3 �l cell suspensions were used for RNA-sequencing (RNA-seq) and ATAC-sequencing (ATAC-seq), respectivelyOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesCharles GirardotEmilia EspositofalseATAC-seq of wild-type mouse PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regionsTo describe chromatin accessibility changes associated with differentiation of mouse presomitic mesoderm (PSM) cells into somites, ATAC sequencing (paired-end) was performed using non-cultured, wild-type PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions. Omni-ATAC protocol was used, and each condition includes six biological replicates.�2025-08-06T00:00:00Z2025-08-07T00:01:25.679Z2024-01-04T14:11:51.262ZE-MTAB-13692ERP156459EFO_0007045EFO_0004170EFO_0005518EFO_0004184