{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Charles Girardot"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-13693"],"description":["To describe gene expression changes associated with differentiation of mouse presomitic mesoderm (PSM) cells into somites, RNA sequencing (paired-end) was performed using non-cultured, wild-type PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions. Smart-seq2 protocol was used, and each condition includes six biological replicates."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - single-end sequencing on illumina NextSeq 500","Growth Protocol - Mice were crossed, and females were dissected 10 days after a detection of vaginal plug, and the PSM tissues isolated.","Growth Protocol - PSM explants of E10.5 wild-type embryos were microdissected into tail bud, posterior PSM, anterior PSM, and somite regions by micro scalpel in cold PBS. Each tissue region was transferred into a micro well (ibidi, #80486) and mechanically dissociated to a cell suspension in 4.2 �l cold PBS. Finally, 0.7 �l and 3.3 �l cell suspensions were used for RNA-sequencing (RNA-seq) and ATAC-sequencing (ATAC-seq), respectively","Library Construction - RNA-seq libraries were prepared following the Smart-seq2 described in Picelli et al., Nat Protoc, 2014"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Charles Girardot","Emilia Esposito"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of wild-type mouse PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions","description":"To describe gene expression changes associated with differentiation of mouse presomitic mesoderm (PSM) cells into somites, RNA sequencing (paired-end) was performed using non-cultured, wild-type PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions. Smart-seq2 protocol was used, and each condition includes six biological replicates.","dates":{"release":"2025-08-06T00:00:00Z","modification":"2025-08-07T00:02:08.294Z","creation":"2024-01-04T14:12:45.261Z"},"accession":"E-MTAB-13693","cross_references":{"ENA":["ERP156460"],"EFO":["EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}